Antibacterial drug-loaded electrospun nano- and microfibrous dressings are of major interest as novel topical drug delivery systems in wound care. In this study, chloramphenicol (CAM)-loaded polycaprolactone (PCL) and PCL/poly(ethylene oxide) (PEO) fiber mats were electrospun and characterized in terms of morphology, drug distribution, physicochemical properties, drug release, swelling, cytotoxicity, and antibacterial activity. Computational modeling together with physicochemical analysis helped to elucidate possible interactions between the drug and carrier polymers. Strong interactions between PCL and CAM together with hydrophobicity of the system resulted in much slower drug release compared to the hydrophilic ternary system of PCL/PEO/CAM. Cytotoxicity studies confirmed safety of the fiber mats to murine NIH 3T3 cells. Disc diffusion assay demonstrated that both fast and slow release fiber mats reached effective concentrations and had similar antibacterial activity. A biofilm formation assay revealed that both blank matrices are good substrates for the bacterial attachment and formation of biofilm. Importantly, prolonged release of CAM from drug-loaded fibers helps to avoid biofilm formation onto the dressing and hence avoids the treatment failure.

中文翻译：

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氯霉素，载体聚合物和细菌之间的相互作用-设计抗静电感染的电纺药物输送系统的意义

载有抗菌药物的电纺纳米纤维敷料和微纤维敷料作为伤口护理中的新型局部药物递送系统引起了人们的极大兴趣。在这项研究中，对氯霉素（CAM）负载的聚己内酯（PCL）和PCL /聚环氧乙烷（PEO）纤维毡进行了电纺丝，并在形态，药物分布，理化特性，药物释放，溶胀，细胞毒性和抗菌活性。计算模型与理化分析一起帮助阐明了药物与载体聚合物之间可能存在的相互作用。与PCL / PEO / CAM的亲水三元系统相比，PCL和CAM之间的强相互作用以及该系统的疏水性导致药物释放慢得多。细胞毒性研究证实了纤维垫对鼠NIH 3T3细胞的安全性。椎间盘扩散试验表明，快速释放纤维垫和缓慢释放纤维垫均达到有效浓度，并具有相似的抗菌活性。生物膜形成试验表明，两种空白基质都是细菌附着和生物膜形成的良好基质。重要的是，CAM从载药纤维中的释放时间长，可避免在敷料上形成生物膜，从而避免治疗失败。