Four new polyketides, penitalarins A–C (1–3) and nafuredin B (4), together with the known biogenetically related nafuredin A (5) were isolated from a mixed culture of a deep-sea-derived fungus Talaromyces aculeatus and a mangrove-derived fungus Penicillium variabile. Liquid chromatography/mass spectrometry analysis showed that none of compounds 1–5 was produced by either of the two fungi when cultured alone under the same condition. The structures of 1–4, including absolute configurations, were deduced based on the interpretation of MS, NMR data, and time-dependent density functional theory calculations of specific electronic circular dichroism spectra. Compounds 1–3 possess a 3,6-dioxabicyclo[3.1.0]hexane ring, and 4 showed cytotoxicity with IC₅₀ values ranging from 1.2 to 9.8 μM against a panel of human cancer cell lines.

中文翻译：

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刺孢菌和变异青霉菌联合培养诱导次生代谢产物的产生

四个新的聚酮化合物，penitalarins A-C（1 - 3）和nafuredin B（4），连同已知biogenetically相关nafuredin A（5）得自深海衍生菌的混合培养物中分离的Talaromyces棘孢和红树来源的真菌青霉变种。液相色谱法/质谱分析表明，没有化合物的1 - 5是由任两个真菌的相同的条件下单独培养时产生的。1 – 4的结构包括绝对构型在内，是根据MS的解释，NMR数据以及特定电子圆二色性光谱随时间变化的密度泛函理论计算得出的。化合物1 - 3具有3,6-二氧杂双环[3.1.0]己烷环，和4与IC表明细胞毒性_50个值范围从1.2至9.8μM对人癌症细胞系的面板。