In environmental engineering and science, fluorescent excitation-emission matrix (EEM) has increasingly been utilized to characterize chromophoric dissolved organic matter (CDOM). This study aims to delineate data processing, including calculation of total fluorescence (TF) which is an emerging water quality parameter often used as a surrogate for trace organic contaminant (TOrC) removal by advanced water treatment processes. In addition, sample handling procedures such as storage, use of preservatives, and oxidant quenching agents were evaluated. In this study, three antimicrobial preservatives were tested: sodium azide, sodium omadine, and thymol. All the tested preservatives altered optical properties of samples, and were therefore not suitable for the preservation of EEM samples. Without preservative, storage of samples at 4 °C maintained TF within 7.5% of its original value for 21 days, while TF of samples stored at the room temperature more drastically changed (up to 15%). The impacts of three oxidant quenching agents including ascorbic acid, sodium bisulfite, and sodium thiosulfate on EEM were also tested. Among the quenching agents, sodium bisulfite was found to be suitable since it little influenced optical properties of samples while the other two were not favorable due to interference. We also scrutinized the use of TF as surrogate to monitor micropollutant rejection by nanofiltration membrane.

在环境工程和科学中，越来越多地利用荧光激发发射矩阵（EEM）来表征发色溶解的有机物（CDOM）。这项研究旨在描述数据处理，包括计算总荧光（TF），这是一种新兴的水质参数，通常用作通过高级水处理工艺去除痕量有机污染物（TOrC）的替代物。此外，还评估了样品处理程序，例如储存，使用防腐剂和氧化剂淬灭剂。在这项研究中，测试了三种抗微生物防腐剂：叠氮化钠，阿魏酸钠和百里酚。所有测试的防腐剂都改变了样品的光学性能，因此不适合用于EEM样品的保存。没有防腐剂 样品在4°C下保存21天的TF保持在其原始值的7.5％以内，而室温下保存的样品的TF发生了较大的变化（高达15％）。还测试了三种氧化剂淬灭剂（包括抗坏血酸，亚硫酸氢钠和硫代硫酸钠）对EEM的影响。在淬灭剂中，发现亚硫酸氢钠是合适的，因为它几乎不影响样品的光学性能，而另外两种由于干扰而不利。我们还详细研究了使用TF作为替代品来监测纳滤膜对微污染物的排斥。还对EEM上的硫代硫酸钠和硫代硫酸钠进行了测试。在淬灭剂中，发现亚硫酸氢钠是合适的，因为它几乎不影响样品的光学性能，而另外两种由于干扰而不利。我们还详细研究了使用TF作为替代品来监测纳滤膜对微污染物的排斥。还对EEM上的硫代硫酸钠和硫代硫酸钠进行了测试。在淬灭剂中，发现亚硫酸氢钠是合适的，因为它几乎不影响样品的光学性能，而另外两种由于干扰而不利。我们还详细研究了使用TF作为替代品来监测纳滤膜对微污染物的排斥。