This manuscript offers a biochemical insight into how the novel strain A. borkumensis can grow effectively on metal-free carbonaceous materials in the presence of hexadecane and BTEX hydrocarbon samples. In the present research, by combination of two eco-friendly processes including microbial-mediated and metal-free and carbonaceous material processes (GO, N-doped reduced graphene oxide, and N–P-co-doped reduced graphene oxide), a green, brand new, and hybrid hydrocarbon degrader was synthesized. In fact, a new enzymatic biodegradation method of petroleum hydrocarbons including hexadecane and BTEX compounds using crude enzymes obtained from Alcanivorax borkumensis in the presence of undoped and simultaneously doped and reduced carbonaceous materials is reported for the first time. The results indicated that higher efficiency of hydrocarbon enzymatic biodegradation was achieved when simultaneously reduced and doped carbonaceous materials were utilized. Studies showed that by using reduced GOs and doping with N and P, their biodegradation efficiencies improved by increasing the hydrophobic adsorption, interfacial area, and bacterial growth/population as well. Overall, the studies also indicated that our results could be considered as a promising approach in this field due to the ability of hydrocarbon removal in high concentrations (up to 8000 mg L⁻¹ for hexadecane) in a short time (7 days). Total protein and enzyme activity assays were evaluated.

中文翻译：

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增强的酶活性和易地石油烃的溶液中使用的生物降解Alcanivorax borkumensis酶在氮和磷的存在下共掺杂还原的石墨烯氧化物作为细菌生长促进剂

该手稿提供了一种生化方法，以了解在存在十六烷和BTEX烃样品的情况下，新型菌株A. borkumensis如何在无金属的碳质材料上有效生长。在本研究中，通过将两种环保工艺（包括微生物介导的无金属碳素材料工艺（GO，N掺杂的还原氧化石墨烯和N-P共掺杂的还原氧化石墨烯））结合起来，绿色合成了全新的杂化烃降解剂。实际上，一种新的酶法降解石油烃（包括十六烷和BTEX化合物）的方法是，使用粗制铝藻获得的粗酶。首次报道了在存在未掺杂，同时掺杂和还原的碳质材料的情况下。结果表明，当同时使用还原和掺杂的碳质材料时，可以实现更高的烃类酶促生物降解效率。研究表明，通过使用减少的GO并掺杂N和P，它们的生物降解效率会通过增加疏水性吸附，界面面积以及细菌的生长/繁殖而得到改善。总体而言，研究还表明，由于在短时间内（7天）以高浓度（十六烷高达8000 mg L ^-1）去除碳氢化合物的能力，我们的结果在该领域可被视为有前途的方法。评估了总蛋白和酶活性测定。