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Atom-Specific Mutagenesis Reveals Structural and Catalytic Roles for an Active-Site Adenosine and Hydrated Mg2+ in Pistol Ribozymes
Angewandte Chemie International Edition ( IF 16.6 ) Pub Date : 2017-11-15 12:00:41 , DOI: 10.1002/anie.201708679
Sandro Neuner 1 , Christoph Falschlunger 1 , Elisabeth Fuchs 1 , Maximilian Himmelstoss 1 , Aiming Ren 2 , Dinshaw J. Patel 3 , Ronald Micura 1
Affiliation  

Smoking gun: Atom-specific mutagenesis revealed that the ribose moiety of a conserved adenosine (A32) and a hydrated Mg2+ ion in the active site of the pistol ribozyme contribute to the cleavage activity. A mechanistic proposal is suggested in which A32 plays a structural role and the hydrated Mg2+ plays a catalytic role in cleavage.

中文翻译:

特定于原子的诱变揭示手枪核酶中活性位点腺苷和水合Mg2 +的结构和催化作用

吸烟枪:原子特异性诱变显示,保守的腺苷(A32)的核糖部分和手枪核酶活性位点中的水合Mg 2+离子有助于裂解活性。提出了一种机制建议,其中A32发挥结构作用,水合Mg 2+在裂解中起催化作用。
更新日期:2017-11-16
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