In this work lipase CaLB was immobilized on functionalized Pd-SiO₂ nanoparticles in order to simplify the DKR of α-methylbenzylamine. Hybrid biocatalysts showed immobilization efficiencies of 82%, 80% and 76% when containing 1, 5 and 10% of Pd respectively. On DKR reaction values of ee > 99% and conversion of 82% were found with only 1% of Pd, generating a productivity of 2.21 mg of product h⁻¹ mg of support⁻¹ against 0.76 found by N435®. Compared to commercial N435®, the novel biocatalysts showed protein loads about 15-fold lower and higher activity, demonstrating competitive performances and high industrial applications.

在这项工作中，脂肪酶CaLB固定在功能化的Pd-SiO ₂纳米颗粒上，以简化α-甲基苄胺的DKR。当分别包含1、5和10％的Pd时，杂化生物催化剂的固定化效率分别为82％，80％和76％。在DKR上，仅使用1％的Pd时，发现ee的反应值> 99％，转化率达到82％，相对于N435®的0.76 ，产生的生产率为2.21 mg产品h ^-1  mg载体^-1。与商业化的N435®相比，新型生物催化剂显示出较低的蛋白质负载量和较高的活性，高出15倍，显示出竞争优势和较高的工业应用价值。