The detection of hypochlorous acid (HOCl) in vivo is vitally important because the local concentration of HOCl is highly correlated with some diseases such as atherosclerosis and rheumatoid arthritis. However, in vivo detection of HOCl remains a challenge due to the lack of a suitable probe. We report here a near-infrared (NIR) emissive “turn-on” probe (FDOCl-1) based on a methylene blue derivative, which can quickly detect HOCl via a newly found deformylation mechanism. FDOCl-1 displays remarkable selectivity and sensitivity towards HOCl. The dramatic changes in colour and NIR emission were used to detect HOCl in vitro and in vivo in a mouse arthritis model.

中文翻译：

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基于脱甲酰反应的探针在体内对HOCl进行成像

体内次氯酸（HOCl）的检测至关重要，因为HOCl的局部浓度与某些疾病（如动脉粥样硬化和类风湿关节炎）高度相关。然而，由于缺乏合适的探针，体内检测HOC1仍然是一个挑战。我们在此报告了一种基于亚甲基蓝衍生物的近红外（NIR）发射“开启”探针（FDOC1-1），该探针可以通过新发现的甲酰基化机制快速检测HOCl 。FDOC1-1对HOC1显示出显着的选择性和敏感性。颜色和NIR发射的剧烈变化用于体外和体内检测HOCl 在小鼠关节炎模型中。