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Biosensor applications of graphene-nanocomposites bound oxidoreductive and hydrolytic enzymes
Analytical Methods ( IF 3.1 ) Pub Date : 2017-11-14 00:00:00 , DOI: 10.1039/c7ay02606d
Qayyum Husain 1, 2, 3, 4, 5
Affiliation  

Graphene based nanocomposites have successfully been employed for high yield and stable immobilization of oxidoreductive and hydrolytic enzymes. Enzymes immobilized both via adsorption and covalent attachment on graphene nanocomposites were found highly stable against various types of denaturants and were noticed less sensitive to inhibition mediated by their specific inhibitors. The oxidoreductases and hydrolases immobilized through graphene nanocomposites have resulted in the formation of robust biocatalysts which have easily been applied in various biosensors on their repeated uses. These nanocomposite bound enzymes have shown very high specificity, selectivity, broad ranges of linearity and sensitivity and very low detection limit. By using such types of techniques several enzymes have successfully been exploited. The results have demonstrated that in many cases the activity of enzymes was remarkably enhanced upon immobilization on such supports. Graphene mediated immobilized enzymes on electrodes have successfully been employed as biosensors for the analysis of various compounds in clinical, environmental, fuel and food samples. The compounds that have been detected by using such kinds of technology are acetylcholine, ethanol, H2O2, c, histamine, catechol, phenol, 4-CP, 3,4-DMP, 3-MP, 4-MP, 4-Cl-3-MP, 4,-Cl-2-MP, caffeic acid, 17β-estradiol, artesunate, CEA, H2S, BPA, captopril, glucose, urea, clenbuterol, Con A, OPs pesticides, paraoxon-ethyl and chlorpyrifos.

中文翻译:

石墨烯-纳米复合物结合的氧化还原酶和水解酶在生物传感器中的应用

石墨烯基纳米复合材料已成功用于氧化还原酶和水解酶的高收率和稳定的固定化。固定化酶均通过发现在石墨烯纳米复合材料上的吸附和共价连接对各种类型的变性剂高度稳定,并且发现它们对由其特定抑制剂介导的抑制作用较不敏感。通过石墨烯纳米复合材料固定化的氧化还原酶和水解酶已导致形成坚固的生物催化剂,这些催化剂很容易重复使用,已广泛应用于各种生物传感器中。这些纳米复合物结合的酶显示出非常高的特异性,选择性,宽范围的线性和灵敏度以及非常低的检测限。通过使用这种类型的技术,已经成功地开发了几种酶。结果表明,在许多情况下,固定在这种支持物上时,酶的活性显着增强。石墨烯介导的固定在电极上的酶已成功地用作生物传感器,用于分析临床,环境,燃料和食品样品中的各种化合物。使用此类技术检测到的化合物为乙酰胆碱,乙醇,H2 O 2,c,组胺,邻苯二酚,苯酚,4-CP,3,4-DMP,3-MP,4-MP,4-Cl-3-MP,4--Cl-2-MP,咖啡酸, 17β-雌二醇,青蒿琥酯,CEA,H 2 S,BPA,卡托普利,葡萄糖,尿素,盐酸克伦特罗,Con A,OPs农药,对氧磷乙基和毒死rif。
更新日期:2017-11-14
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