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Early Metabolome Profiling and Prognostic Value in Paraquat-Poisoned Patients: Based on Ultraperformance Liquid Chromatography Coupled To Quadrupole Time-of-Flight Mass Spectrometry
Chemical Research in Toxicology ( IF 4.1 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1021/acs.chemrestox.7b00240
Lufeng Hu 1 , Guangliang Hong 2 , Yahui Tang 2 , Xianqin Wang 3 , Congcong Wen 3 , Feiyan Lin 4 , Zhongqiu Lu 2
Affiliation  

Paraquat (PQ) has caused countless deaths throughout the world. There remains no effective treatment for PQ poisoning. Here we study the blood metabolome of PQ-poisoned patients using ultraperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS). Patients were divided into groups according to blood PQ concentration. Healthy subjects served as controls. Metabolic features were statistically analyzed using multivariate pattern-recognition techniques to identify the most important metabolites. Selected metabolites were further compared with a series of clinical indexes to assess the prognostic value. PQ-poisoned patients showed substantial differences compared with healthy subjects. Based on variable of importance in the project (VIP) values and statistical analysis, 17 metabolites were selected and identified. These metabolites well-classified low PQ-poisoned patients, high PQ-poisoned patients, and healthy subjects, which was better than that of a complete blood count (CBC). Among the 17 metabolites, 20:3/18:1-PC (PC), LPA (0:0/16:0) (LPA), 19-oxo-deoxycorticosterone (19-oxo-DOC), and eicosapentaenoic acid (EPA) had prognostic value. In particular, EPA was the most sensitive one. Besides, the levels of EPA was correlated with LPA and 19-oxo-DOC. If EPA was excessively consumed, then prognosis was poor. In conclusion, the serum metabolome is substantially perturbed by PQ poisoning. EPA is the most important biomarker in early PQ poisoning.

中文翻译:

百草枯中毒患者的早期代谢组谱分析和预后价值:基于超高效液相色谱和四极杆飞行时间质谱联用

百草枯(PQ)在全世界造成了无数的死亡。目前尚无有效的PQ中毒治疗方法。在这里,我们使用超高效液相色谱结合四极杆飞行时间质谱(UPLC / Q-TOF MS)研究PQ中毒患者的血液代谢组。根据血液中PQ浓度将患者分为几组。健康受试者作为对照。使用多元模式识别技术对代谢特征进行统计分析,以鉴定最重要的代谢物。进一步将选定的代谢物与一系列临床指标进行比较,以评估预后价值。与健康受试者相比,PQ中毒的患者表现出显着差异。根据项目中的重要变量(VIP)值和统计分析,选择并鉴定了17种代谢物。这些代谢物对低PQ中毒的患者,高PQ中毒的患者和健康受试者进行了分类,优于全血细胞计数(CBC)。在17种代谢产物中,20:3/18:1-PC(PC),LPA(0:0/16:0)(LPA),19-氧代-脱氧皮质酮(19-氧代-DOC)和二十碳五烯酸(EPA) )具有预后价值。尤其是EPA是最敏感的一种。此外,EPA的水平与LPA和19-oxo-DOC有关。如果EPA消耗过多,则预后很差。总之,PQ中毒会严重干扰血清代谢组。EPA是早期PQ中毒中最重要的生物标志物。比全血细胞计数(CBC)更好。在17种代谢产物中,20:3/18:1-PC(PC),LPA(0:0/16:0)(LPA),19-氧代-脱氧皮质酮(19-氧代-DOC)和二十碳五烯酸(EPA) )具有预后价值。尤其是EPA是最敏感的一种。此外,EPA的水平与LPA和19-oxo-DOC有关。如果EPA消耗过多,则预后很差。总之,PQ中毒会严重干扰血清代谢组。EPA是早期PQ中毒中最重要的生物标志物。比全血细胞计数(CBC)更好。在17种代谢产物中,20:3/18:1-PC(PC),LPA(0:0/16:0)(LPA),19-氧代-脱氧皮质酮(19-氧代-DOC)和二十碳五烯酸(EPA) )具有预后价值。尤其是EPA是最敏感的一种。此外,EPA的水平与LPA和19-oxo-DOC有关。如果EPA消耗过多,则预后很差。总之,PQ中毒会严重干扰血清代谢组。EPA是早期PQ中毒中最重要的生物标志物。那么预后很差。总之,PQ中毒会严重干扰血清代谢组。EPA是早期PQ中毒中最重要的生物标志物。那么预后很差。总之,PQ中毒会严重干扰血清代谢组。EPA是早期PQ中毒中最重要的生物标志物。
更新日期:2017-11-13
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