Herein, bimetallic core-shell [email protected] nanocrystals ([email protected] NCs) were prepared by a simple one-pot aqueous method using 2-pyrrolidone-5-carboxylic acid sodium salt (PCA-Na) as a new and green growth-directing agent. The obtained architectures displayed excellent catalytic activity towards oxygen reduction reaction (ORR) compared with commercial Pt/C catalyst. A novel immunosensor was constructed via assembly prostate specific antibody on the surface of [email protected] NCs. It was found that the ORR currents were significantly suppressed due to the specific antigen-antibody reaction. The ultra-sensitive determination of prostate specific antigen (PSA) was realized on account of the immunocomplex impeding the redox probe accessible to the electrode. The immunosensor exhibited good analytical performance for the assay of PSA with the wide linear range of 0.1 ~ 50 ng mL^–1 and low detection limit of 0.018 ng mL^–1 (S/N = 3), coupled with the improved stability, reproducibility and selectivity.

在此，使用2-吡咯烷酮-5-羧酸钠盐（PCA-Na）作为新的绿色生长物，通过简单的一锅法制备双金属核壳[受电子邮件保护的] NC晶体（[受电子邮件保护的] NC）。导演。与商业化的Pt / C催化剂相比，所获得的体系结构对氧还原反应（ORR）表现出优异的催化活性。一种新型的免疫传感器是通过在[受电子邮件保护的] NC的表面上装配前列腺特异性抗体。发现由于特异性抗原-抗体反应而显着抑制了ORR电流。由于免疫复合物阻碍了电极上的氧化还原探针的接触，因此实现了前列腺特异性抗原（PSA）的超灵敏测定。该免疫传感器对PSA的分析显示出良好的分析性能，线性范围为0.1〜50 ng mL ^–1，检测下限为0.018 ng mL ^–1（S / N = 3），并具有更高的稳定性，重现性和选择性。