Hydrogen sulfide (H₂S) is an endogenous modulator that plays significant physio-pathological roles in several biological systems. In this research field there is a large interest in developing selective CBS and CSE inhibitors and H₂S releasing moieties, that could be either used as therapeutic agents or linked to known drugs. One of the major problem is the limited availability of chemicals that ensure a controlled release of H₂S in vitro as well in vivo. Aiming to obtain novel H₂S donors, whose release properties could be appropriately modulated, we have synthesized a series of 1,2,4-thiadiazolidine-3,5-diones (THIA 1–10) as innovative donors that could release H₂S in controlled manner. All the synthesized compounds were evaluated for their H₂S releasing properties by an amperometric approach and for their vasorelaxant ability on aorta rings. In order to rationalize the obtained results, a detailed study on the release mechanism has been performed using the most efficient H₂S donor, THIA 3 (C_max 65.4 μM and EC₅₀ 1.7 μM).

硫化氢（H ₂ S）是一种内源性调节剂，在几种生物系统中起着重要的生理病理作用。在该研究领域中，人们对开发选择性的CBS和CSE抑制剂以及H ₂ S释放部分非常感兴趣，它们既可以用作治疗剂，也可以与已知药物连接。主要问题之一是确保体外和体内H ₂ S受控释放的化学药品的供应有限。为了获得可以适当调节释放特性的新型H ₂ S供体，我们合成了一系列1,2,4-噻二唑烷-3,5-二酮（THIA 1-10）作为可以释放H _2的创新性供体。S以受控方式。通过电流分析法评估所有合成的化合物的H ₂ S释放特性，以及对主动脉环的血管舒张能力。为了使获得的结果合理化，已使用最有效的H ₂ S供体THIA 3（C _max 65.4μM和EC ₅₀ 1.7μM）对释放机理进行了详细研究。