Direct revegetation, or phytostabilization, is a containment strategy for contaminant metals associated with mine tailings in semiarid regions. The weathering of sulfide ore-derived tailings frequently drives acidification that inhibits plant establishment resulting in materials prone to wind and water dispersal. The specific objective of this study was to associate pyritic mine waste acidification, characterized through pore-water chemistry analysis, with dynamic changes in microbial community diversity and phylogenetic composition, and to evaluate the influence of different treatment strategies on the control of acidification dynamics. Samples were collected from a highly instrumented one-year mesocosm study that included the following treatments: 1) unamended tailings control; 2) tailings amended with 15% compost; and 3) the 15% compost-amended tailings planted with Atriplex lentiformis. Tailings samples were collected at 0, 3, 6 and 12 months and pore water chemistry was monitored as an indicator of acidification and weathering processes. Results confirmed that the acidification process for pyritic mine tailings is associated with a temporal progression of bacterial and archaeal phylotypes from pH sensitive Thiobacillus and Thiomonas to communities dominated by Leptospirillum and Ferroplasma. Pore-water chemistry indicated that weathering rates were highest when Leptospirillum was most abundant. The planted treatment was most successful in disrupting the successional evolution of the Fe/S-oxidizing community. Plant establishment stimulated growth of plant-growth-promoting heterotrophic phylotypes and controlled the proliferation of lithoautotrophic Fe/S-oxidizers. The results suggest the potential for eco-engineering a microbial inoculum to stimulate plant establishment and inhibit proliferation of the most efficient Fe/S-oxidizing phylotypes.

直接植被恢复或植物稳定化是针对与半干旱地区矿山尾矿有关的污染物金属的遏制策略。硫化物矿石尾矿的风化经常会驱动酸化作用，从而抑制植物的生长，从而导致材料易于风和水分散。这项研究的具体目的是将通过孔隙水化学分析表征的黄铁矿废料酸化与微生物群落多样性和种系组成的动态变化联系起来，并评估不同处理策略对酸化动力学控制的影响。从一项高度仪器化的为期一年的中观研究中收集样品，该研究包括以下处理：1）未修正的尾矿对照；2）尾矿中添加了15％的堆肥；三角滨藜。分别在0、3、6和12个月收集尾矿样品，并监测孔隙水的化学性质，作为酸化和风化过程的指标。结果证实，对于黄铁矿尾矿酸化过程与来自pH敏感的细菌和古细菌的种系型的时间进展相关硫杆菌属和Thiomonas由主导社区钩端螺旋体和铁原体。孔隙水化学表明，钩端螺旋体风化率最高是最丰富的。种植的处理最成功地破坏了Fe / S氧化群落的连续进化。植物的建立刺激了促进植物生长的异养系统型的生长，并控制了自养岩石Fe / S-氧化剂的增殖。结果表明，生态工程改造微生物接种物有可能刺激植物的生长并抑制最有效的Fe / S氧化系统型的繁殖。