AbstractThis paper describes a CdTe quantum dot-based fluorescence resonance energy transfer (FRET) based assay for the detection of the breast cancer biomarker microRNA. The method relies on energy transfer between DNA-templated silver nanoclusters (AgNCs) and CdTe QDs. Interaction between double strand oligonucleotide and QDs can be detected qualitatively through gel analysis and quantitatively by the signal amplification from AgNCs to QDs via FRET, best measured at an excitation wavelength of 350 nm and at emission wavelengths of 550 and 590 nm. Three microRNAs (microRNA-21, microRNA-155 and Let-7a) were quantified to verify the feasibility of the method, and a high sensitivity for microRNAs was achieved. Fluorescence intensity increases linearly with the log of the concentration of microRNA 155 in the 5.0 pM to 50 nM range, with a 1.2 pM detection limit. Graphical abstractSchematic presentation of a quantum dot-based (QD-based) fluorescence resonance energy transfer technique for the detection of microRNA (miRNA). The method relies on energy transfer between DNA-templated silver nanoclusters (AgNCs) and QDs.

中文翻译：

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通过 FRET 在银纳米团簇和 CdTe 量子点之间荧光测定 microRNA

摘要本文描述了一种基于 CdTe 量子点的荧光共振能量转移 (FRET) 检测方法，用于检测乳腺癌生物标志物 microRNA。该方法依赖于 DNA 模板化银纳米团簇 (AgNC) 和 CdTe QD 之间的能量转移。双链寡核苷酸和量子点之间的相互作用可以通过凝胶分析定性检测，并通过 FRET 从 AgNCs 到量子点的信号放大进行定量检测，最好在 350 nm 的激发波长和 550 和 590 nm 的发射波长下测量。对三种 microRNA（microRNA-21、microRNA-155 和 Let-7a）进行定量以验证该方法的可行性，并实现了 microRNA 的高灵敏度。在 5.0 pM 至 50 nM 范围内，荧光强度随 microRNA 155 浓度的对数线性增加，值为 1。2 pM 检测限。用于检测微小RNA (miRNA) 的基于量子点（基于QD）的荧光共振能量转移技术的示意图。该方法依赖于 DNA 模板化银纳米团簇 (AgNC) 和 QD 之间的能量转移。