Viruses accomplish their replication by exploiting many cellular resources, including metabolites and energy. Similarly to other (+)RNA viruses, tomato bushy stunt virus (TBSV) induces major changes in infected cells. However, the source of energy required to fuel TBSV replication is unknown. We find that TBSV co-opts the cellular glycolytic ATP-generating pyruvate kinase (PK) directly into the viral replicase complex to boost progeny RNA synthesis. The co-opted PK generates high levels of ATP within the viral replication compartment at the expense of a reduction in cytosolic ATP pools. The ATP generated by the co-opted PK is used to promote the helicase activity of recruited cellular DEAD-box helicases, which are involved in the production of excess viral (+)RNA progeny. Altogether, recruitment of PK and local production of ATP within the replication compartment allow the virus replication machinery an access to plentiful ATP, facilitating robust virus replication.

病毒通过利用许多细胞资源（包括代谢物和能量）来完成其复制。与其他（+）RNA病毒相似，番茄丛状特技病毒（TBSV）诱导感染细胞发生重大变化。但是，为TBSV复制提供动力所需的能源尚不清楚。我们发现，TBSV将细胞糖酵解ATP产生的丙酮酸激酶（PK）直接加入病毒复制酶复合物中，以增强子代RNA的合成。共同选择的PK在病毒复制区室中产生高水平的ATP，但以减少胞质ATP池为代价。由共选PK产生的ATP用于促进募集的细胞DEAD-box解旋酶的解旋酶活性，这些酶参与了过量病毒（+）RNA后代的产生。共，