Double take: We show that a “bootstrapped” biocatalysis substrate material can both purify and immobilize multiple enzymes site specifically and directly from crude cell lysates. We demonstrate the utility of the technique in a bienzymatic stereoselective reduction coupled with a cofactor recycling scheme. The domains allow for several cycles of selective removal and replacement of enzymes under mild conditions to regenerate the catalyst system.

中文翻译：

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自举式生物催化：生物膜衍生的材料作为可逆功能化的多酶表面

双重注意：我们证明了“自举”生物催化底物材料既可以纯化也可以直接从粗细胞裂解物中固定多种酶。我们证明了该技术在双酶立体选择性还原结合辅因子回收方案中的实用性。该结构域允许在温和条件下选择性去除和置换酶的多个循环，以再生催化剂体系。