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Modulation of cellular polarization and migration by ephrin/Eph signal-mediated boundary formation
Integrative Biology ( IF 2.5 ) Pub Date : 2017-11-02 00:00:00 , DOI: 10.1039/c7ib00176b
Sahar Javaherian 1, 2, 3, 4 , Elisa D’Arcangelo 2, 4, 5 , Benjamin Slater 1, 2, 3, 4 , Camila Londono 2, 4, 5 , Bin Xu 2, 4, 5 , Alison P. McGuigan 1, 2, 3, 4, 5
Affiliation  

Compartment boundaries are essential for ensuring proper cell organization during embryo development and in adult tissues, yet the mechanisms underlying boundary establishment are not completely understood. A number of mechanisms, including (i) differential adhesion, (ii) differential tension, and (iii) cell signaling-mediated cell repulsion, are known to contribute and likely a context-dependent balance of each of these dictates boundary implementation. The ephrin/Eph signaling pathway is known to impact boundary formation in higher animals. In different contexts, ephrin/Eph signaling is known to modulate adhesive properties and migratory behavior of cells. Furthermore it has been proposed that ephrin/Eph signaling may modulate cellular tensile properties, leading to boundary implementation. It remains unclear however, whether, in different contexts, ephrin/Eph act through distinct dominant action modes (e.g. differential adhesion vs. cell repulsion), or whether ephrin/Eph signaling elicits multiple cellular changes simultaneously. Here, using micropatterning of cells over-expressing either EphB3 or ephrinB1, we assess the contribution of each these factors in one model. We show that in this system ephrinB1/EphB3-mediated boundaries are accompanied by modulation of tissue-level architecture and polarization of cell migration. These changes are associated with changes in cell shape and cytoskeletal organization also suggestive of altered cellular tension.

中文翻译:

ephrin / Eph信号介导的边界形成对细胞极化和迁移的调节

隔室边界对于确保胚胎发育期间和成年组织中的适当细胞组织至关重要,但尚未完全理解边界建立的机制。已知许多机制,包括(i)差异粘附,(ii)差异张力和(iii)细胞信号传导介导的细胞排斥,可能会影响这些机制,并且可能取决于上下文的平衡决定了边界的实现。ephrin / Eph信号通路已知会影响高等动物的边界形成。在不同的情况下,已知ephrin / Eph信号调节细胞的粘附特性和迁移行为。此外,已经提出了ephrin / Eph信号传导可以调节细胞的拉伸特性,从而导致边界的实现。但是,目前尚不清楚(例如差异粘附细胞排斥),或者ephrin / Eph信号传导是否同时引发多个细胞变化。在这里,我们使用过度表达EphB3或ephrinB1的细胞的微模式,我们评估了每个因素在一个模型中的贡献。我们表明,在该系统中,ephrinB1 / EphB3介导的边界伴随着组织水平结构的调节和细胞迁移的极化。这些变化与细胞形状和细胞骨架组织的变化有关,也暗示了细胞张力的改变。
更新日期:2017-11-09
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