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An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range†
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-11-08 00:00:00 , DOI: 10.1039/c7lc01070b
Takashi Anazawa 1, 2, 3 , Motohiro Yamazaki 3, 4, 5
Affiliation  

Although multi-point, multi-color fluorescence-detection systems are widely used in various sciences, they would find wider applications if they are miniaturized. Accordingly, an ultra-small, four-emission-point and four-color fluorescence-detection system was developed. Its size (space between emission points and a detection plane) is 15 × 10 × 12 mm, which is three-orders-of-magnitude smaller than that of a conventional system. Fluorescence from four emission points with an interval of 1 mm on the same plane was respectively collimated by four lenses and split into four color fluxes by four dichroic mirrors. Then, a total of sixteen parallel color fluxes were directly input into an image sensor and simultaneously detected. The emission-point plane and the detection plane (the image-sensor surface) were parallel and separated by a distance of only 12 mm. The developed system was applied to four-capillary array electrophoresis and successfully achieved Sanger DNA sequencing. Moreover, compared with a conventional system, the developed system had equivalent high fluorescence-detection sensitivity (lower detection limit of 17 pM dROX) and 1.6-orders-of-magnitude higher dynamic range (4.3 orders of magnitude).

中文翻译:

具有高灵敏度和高动态范围的超小型,多点,多色光电检测系统

尽管多点,多色荧光检测系统已广泛应用于各种科学领域,但如果将其小型化,它们会发现更广泛的应用。因此,开发了超小型的四发射点四色荧光检测系统。它的大小(发射点和检测平面之间的空间)为15×10×12 mm,比常规系统小三倍。来自同一平面上间隔为1 mm的四个发射点的荧光分别通过四个透镜进行准直,并通过四个二向色镜分成四个色通量。然后,将总共十六个平行色通量直接输入到图像传感器中并同时进行检测。发射点平面和检测平面(图像传感器表面)平行且相隔仅12 mm的距离。开发的系统应用于四毛细管阵列电泳,并成功实现了Sanger DNA测序。此外,与常规系统相比,该开发的系统具有同等的高荧光检测灵敏度(较低的检测限17 pM dROX)和1.6数量级的较高动态范围(4.3个数量级)。
更新日期:2017-11-08
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