As a proof of principle, a selected reaction monitoring (SRM) mass spectrometry-based methodology was applied to the simultaneous quantification of dozens of protein biomarkers in caged amphipods (Gammarus fossarum). We evaluated the suitability of the methodology to assess complex field contaminations through its application in the framework of a regional river monitoring network. Thanks to the high throughput acquisition of biomarker levels in G. fossarum exposed in four reference and 13 contaminated sites, we analyzed the individual responses of 38 peptides reporting for 25 proteins of interest in 170 organisms. Responses obtained in contaminated sites included inductions of vitellogenin-like proteins in male organisms, inductions of Na⁺K⁺/ATPases, and strong inhibitions of molt-related proteins such as chitinase and JHE-carboxylesterase. Proteins from detoxification and immunity processes were also found modulated in abundance. Summarizing, the results presented here show that the SRM strategy developed for multibiomarker measurement paves a very promising way to define multiple indicators of the health status of sentinel organisms for environmental hazard assessment.

中文翻译：

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用于水生环境监测的生态毒物蛋白质组学：使用笼状两栖动物的基于蛋白质组学的新型生物标记物测定方法的首次现场应用

作为原理的证明，将基于选择的反应监测（SRM）质谱的方法应用于笼状两足动物（Gammarus fossarum）中数十种蛋白质生物标志物的同时定量。我们通过将其应用到区域河流监测网络的框架中，评估了该方法用于评估复杂现场污染的适用性。得益于在四个参考点和13个受污染位点中暴露的G草中生物标志物水平的高通量获取，我们分析了170个生物体中报告的25种感兴趣蛋白的38种肽的个体应答。在受污染部位获得的反应包括雄性生物体中卵黄蛋白原样蛋白的诱导，Na ⁺ K ^+的诱导/ ATPase，并强烈抑制与蜕皮相关的蛋白（如几丁质酶和JHE-羧酸酯酶）。还发现来自排毒和免疫过程的蛋白质被大量调节。总而言之，此处给出的结果表明，为多生物标记物测量开发的SRM策略为定义前哨生物的健康状况的多种指标提供了一种非常有希望的方法，用于环境危害评估。