当前位置: X-MOL 学术Nat. Plants › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Transcriptome dynamics revealed by a gene expression atlas of the early Arabidopsis embryo.
Nature Plants ( IF 18.0 ) Pub Date : 2017-Nov-01 , DOI: 10.1038/s41477-017-0035-3
Joakim Palovaara , Shunsuke Saiga , Jos R. Wendrich , Nicole van ‘t Wout Hofland , J. Paul van Schayck , Friederike Hater , Sumanth Mutte , Jouke Sjollema , Mark Boekschoten , Guido J. Hooiveld , Dolf Weijers

During early plant embryogenesis, precursors for all major tissues and stem cells are formed. While several components of the regulatory framework are known, how cell fates are instructed by genome-wide transcriptional activity remains unanswered-in part because of difficulties in capturing transcriptome changes at cellular resolution. Here, we have adapted a two-component transgenic labelling system to purify cell-type-specific nuclear RNA and generate a transcriptome atlas of early Arabidopsis embryo development, with a focus on root stem cell niche formation. We validated the dataset through gene expression analysis, and show that gene activity shifts in a spatio-temporal manner, probably signifying transcriptional reprogramming, to induce developmental processes reflecting cell states and state transitions. This atlas provides the most comprehensive tissue- and cell-specific description of genome-wide gene activity in the early plant embryo, and serves as a valuable resource for understanding the genetic control of early plant development.

中文翻译:

拟南芥早期胚胎的基因表达图谱揭示了转录组动力学。

在植物早期胚胎发生期间,形成了所有主要组织和干细胞的前体。尽管调节框架的几个组成部分是已知的,但是如何通过全基因组转录活性指导细胞命运的方法仍未得到解答-部分是由于难以以细胞分辨率捕获转录组变化。在这里,我们已经采用了两组分转基因标记系统,以纯化细胞类型特异性核RNA,并生成拟南芥早期胚胎发育的转录组图谱,重点是根干细胞生态位的形成。我们通过基因表达分析验证了数据集,并表明基因活性以时空方式变化,可能表示转录重编程,从而诱导了反映细胞状态和状态转变的发育过程。
更新日期:2017-11-06
down
wechat
bug