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Electrochemical Studies of Ethoxyquin and its Determination in Salmon Samples by Flow Injection Analysis with an Amperometric Dual Detector
Electroanalysis ( IF 3 ) Pub Date : 2017-11-06 , DOI: 10.1002/elan.201700611
Marie Vandeput 1 , Rocío Rodríguez-Gómez 1 , Ange-Michaëlla Izere 1 , Alberto Zafra-Gómez 2 , Kris De Braekeleer 1 , Cédric Delporte 3 , Pierre Van Antwerpen 3 , Jean-Michel Kauffmann 1
Affiliation  

Ethoxyquin (EQ) is an antioxidant widely used in the food industry. Some concerns for human health have been reported since its utilization in animal feed may lead to residues in human food such as salmon samples. This work aimed to investigate the electrochemical behavior of EQ and its major oxidation products namely a dimer of EQ (EQDM) and ethoxyquin quinone imine (EQI) by cyclic voltammetry (CV) at a carbon screen printed electrode (cSPE). The stability of these products and their reactivity against glutathione (GSH) were also studied. Oxidized products and thiol adducts were identified by a microelectrolysis of an EQ solution onto a cSPE in the absence and in the presence of GSH. The products were analyzed off-line, by liquid chromatography coupled to a mass spectrometer (LC-MS). One of the generated product (EQI) was shown to be highly reactive towards GSH. Based on the redox pattern of EQ, a flow injection analysis with a dual cSPE was developed in order to detect in a rapid manner EQ in salmon samples. Since matrix effects were observed, matrix-matched calibration curves with spiked samples were built. A linear response was obtained between 20–100 μM and a limit of detection (LOD) of 7.5 μM (8.2 mg/kg of salmon). Trueness was assessed with recovery assays at three levels of concentration. The recovery was close to 100 %. Precision was determined as RSD (%) with values lower than 6 % in all cases.

中文翻译:

使用电流分析双检测器流动注射分析三文鱼样品中乙氧基喹啉的电化学研究及其测定

Ethoxyquin (EQ) 是一种广泛用于食品工业的抗氧化剂。由于其在动物饲料中的使用可能会导致人类食物(如鲑鱼样本)中的残留,因此已经报告了对人类健康的一些担忧。本工作旨在通过循环伏安法 (CV) 在碳丝网印刷电极 (cSPE) 上研究 EQ 及其主要氧化产物,即 EQ 二聚体 (EQDM) 和乙氧基醌亚胺 (EQI) 的电化学行为。还研究了这些产品的稳定性及其对谷胱甘肽 (GSH) 的反应性。在不存在和存在 GSH 的情况下,通过将 EQ 溶液微电解到 cSPE 上来鉴定氧化产物和硫醇加合物。通过与质谱联用的液相色谱法(LC-MS)离线分析产物。一种生成的产物 (EQI) 显示对 GSH 具有高度反应性。基于 EQ 的氧化还原模式,开发了使用双 cSPE 的流动注射分析,以便快速检测鲑鱼样品中的 EQ。由于观察到了基质效应,因此建立了加标样品的基质匹配校准曲线。在 20–100 μM 和 7.5 μM(8.2 mg/kg 鲑鱼)的检测限 (LOD) 之间获得线性响应。用三个浓度水平的回收试验评估真实度。回收率接近 100%。精密度被确定为 RSD (%),在所有情况下值都低于 6%。建立了加标样品的基质匹配校准曲线。在 20–100 μM 和 7.5 μM(8.2 mg/kg 鲑鱼)的检测限 (LOD) 之间获得线性响应。用三个浓度水平的回收试验评估真实度。回收率接近 100%。精密度被确定为 RSD (%),在所有情况下值都低于 6%。建立了加标样品的基质匹配校准曲线。在 20–100 μM 和 7.5 μM(8.2 mg/kg 鲑鱼)的检测限 (LOD) 之间获得线性响应。用三个浓度水平的回收试验评估真实度。回收率接近 100%。精密度被确定为 RSD (%),在所有情况下值都低于 6%。
更新日期:2017-11-06
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