In this work targeted (selected reaction monitoring, SRM, PASSEL: PASS00697) and panoramic (shotgun LC–MS/MS, PRIDE: PXD00244) mass-spectrometric methods as well as transcriptomic analysis of the same samples using RNA-Seq and PCR methods (SRA experiment IDs: SRX341198, SRX267708, SRX395473, SRX390071) were applied for quantification of chromosome 18 encoded transcripts and proteins in human liver and HepG2 cells. The obtained data was used for the estimation of quantitative mRNA-protein ratios for the 275 genes of the selected chromosome in the selected tissues. The impact of methodological limitations of existing analytical proteomic methods on gene-specific mRNA–protein ratios and possible ways of overcoming these limitations for detection of missing proteins are also discussed.

中文翻译：

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为什么在人类18号染色体上的275个预测的蛋白质编码基因中，mRNA与蛋白质水平之间的相关性如此之低？

在这项工作中，有针对性的（选定的反应监测，SRM，PASSEL：PASS00697）和全景式（shot弹枪LC-MS / MS，PRIDE：PXD00244）质谱法，以及使用RNA-Seq和PCR方法对相同样品进行转录组分析（ SRA实验编号：SRX341198，SRX267708，SRX395473，SRX390071）用于量化人肝和HepG2细胞中18号染色体编码的转录本和蛋白质。获得的数据用于估计所选组织中所选染色体的275个基因的定量mRNA-蛋白质比率。还讨论了现有分析蛋白质组学方法的方法学局限性对基因特异性mRNA /蛋白质比率的影响，以及克服这些局限性以检测缺失蛋白的可能方法。