Chlorophenols (CPs), nitrophenols (NPs), and sulfonamide antibiotics (SAs) are three types of environmental pollutants that are of great concern because of their prevalence and toxicity. In this study, three laccase isoenzymes obtained from Pleurotus ostreatus HAUCC 162 were heterologously expressed and characterized with respect to their ability to degrade CPs, NPs, and, SAs. The three recombinant laccases can efficiently degrade the three types of considered pollutants using a laccase-mediator system (LMS). Their specific efficiencies for the removal of 2NP, 3NP, 4NP, 4CP, 2,4-dichlorophenol (DCP), 2,6-DCP, sulfadiazine (SDZ), sulfamethazine (SMZ), and sulfamethoxazole (SMX) over 60 min were 59.21%, 47.91%, 60.24%, 74.9%, 28.9%, 35.1%, 98.1%, 97.5%, and 97.8%, respectively. Based on the analysis of the oxidation products of the CPs, NPs, and SAs, pollutant removal pathways are proposed, namely, the production of 3-nitromuconate and 3-chloromuconate as the key intermediates of 4-NP and 2, 4-DCP; and oxidative coupling for the transformation of SDZ by LMS. The results of present work indicated the laccases could efficiently remove NPs, CPs, and SAs in LMS, which offers an opportunity to apply P. ostreatus HAUCC 162 laccase in the field of environmental biotechnology.

中文翻译：

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三种平菇HAUCC 162获得的3种漆酶的异源表达与表征。

氯酚（CPs），硝基酚（NPs）和磺酰胺类抗生素（SAs）是三种环境污染物，由于它们的普遍性和毒性而备受关注。在这项研究中，从平菇中获得了三种漆酶同工酶。HAUCC 162异质表达和表征其降解CP，NP和SA的能力。这三种重组漆酶可以使用漆酶介体系统（LMS）有效降解三种考虑的污染物。在60分钟内去除2NP，3NP，4NP，4CP，2,4-二氯苯酚（DCP），2,6-DCP，磺胺嘧啶（SDZ），磺胺二嗪（SMZ）和磺胺甲恶唑（SMX）的比效率为59.21分别为％，47.91％，60.24％，74.9％，28.9％，35.1％，98.1％，97.5％和97.8％。在对CP，NP和SA的氧化产物进行分析的基础上，提出了污染物去除途径，即3-硝基粘康酸酯和3-氯粘康酸酯的生产是4-NP和2,4-DCP的关键中间体。氧化偶联用于LMS转化SDZ。在环境生物技术领域中的P. ostreatus HAUCC 162漆酶。