Thirty-two (32) bis-indolylmethane-hydrazone hybrids 1–32 were synthesized and characterized by ¹HNMR, ¹³CNNMR and HREI-MS. All compounds were evaluated in vitro for β-glucuronidase inhibitory potential. All analogs showed varying degree of β-glucuronidase inhibitory potential ranging from 0.10 ± 0.01 to 48.50 ± 1.10 μM when compared with the standard drug d-saccharic acid-1,4-lactone (IC₅₀ value 48.30 ± 1.20 μM). Derivatives 1–32 showed the highest β-glucuronidase inhibitory potentials which is many folds better than the standard drug d-saccharic acid-1,4-lactone. Further molecular docking study validated the experimental results. It was proposed that bis-indolylmethane may interact with some amino acid residues located within the active site of β-glucuronidase enzyme. This study has culminated in the identification of a new class of potent β-glucuronidase inhibitors.

三十二（32）双-indolylmethane -腙杂交1 - 32被合成和表征通过¹ HNMR，¹³ CNNMR和HREI-MS。在体外评估所有化合物的β-葡萄糖醛酸苷酶抑制潜力。所有类似物表现出不同程度的β葡萄糖醛酸酶抑制潜力范围从0.10±0.01至48.50±1.10  μ当与标准药物相比中号d -saccharic酸-1,4-内酯（IC ₅₀值48.30±1.20  μ M）。导数1–32显示出最高的β-葡糖醛酸糖苷酶的抑制潜力比标准药物d-蔗糖-1,4-内酯要好很多倍。进一步的分子对接研究验证了实验结果。有人提出双吲哚甲烷可能与位于β-葡萄糖醛酸苷酶的活性位点内的一些氨基酸残基相互作用。这项研究最终确定了新型的有效β-葡萄糖醛酸苷酶抑制剂。