当前位置: X-MOL 学术Mater. Chem. Front. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Ground-state conformers enable bright single-fluorophore ratiometric thermometers with positive temperature coefficients
Materials Chemistry Frontiers ( IF 7 ) Pub Date : 2017-08-28 00:00:00 , DOI: 10.1039/c7qm00345e
Weijie Chi 1, 2 , Wenting Yin 3, 4, 5, 6, 7 , Qingkai Qi 3, 4, 5, 6, 7 , Qinglong Qiao 3, 4, 5, 6, 7 , Yuyan Lin 2, 8 , Zhuohui Zhu 2, 8 , Sindhu Vijayan 1, 2 , Michinao Hashimoto 1, 2 , Gayathri Udayakumar 2, 8 , Zhaochao Xu 3, 4, 5, 6, 7 , Xiaogang Liu 1, 2, 2, 8
Affiliation  

Fluorescence thermometry based on organic dyes affords high spatial and temporal resolution with a simple system design and low cost, for measuring temperatures in microenvironments. Many fluorescent thermometers consist of two types of fluorophores with distinct temperature responses, and the ratios of their fluorescence intensities afford accurate temperature information. Yet, the reliability of these ratiometric thermometers is vulnerable to photobleaching induced system variations. In this paper, we have proposed and demonstrated a new strategy, to achieve ratiometric temperature measurements from 15 °C to 75 °C, based on ground-state conformational isomers of a single type of dye. These ground-state conformers emit bright fluorescence, in contrast to excited-state conformational changes that generally quench emissions. Moreover, thermal equilibrium of these conformers and their distinct spectra lead to ratiometric temperature readings that are not affected by photobleaching. We expect that our design strategy has significant implications for developing fluorescence thermometry with outstanding reliability.

中文翻译:

基态规整器可实现具有正温度系数的明亮的单荧光比率式温度计

基于有机染料的荧光测温法可提供高空间和时间分辨率,并具有简单的系统设计和低成本,可用于测量微环境中的温度。许多荧光温度计由两种具有不同温度响应的荧光团组成,它们的荧光强度之比可提供准确的温度信息。然而,这些比例温度计的可靠性易受光漂白引起的系统变化的影响。在本文中,我们提出并演示了一种新策略,该方法基于一种染料的基态构象异构体,实现从15°C到75°C的比例温度测量。与通常使发射猝灭的激发态构象变化相反,这些基态构象子发出明亮的荧光。而且,这些构象异构体的热平衡及其独特的光谱导致比例温度读数不受光漂白的影响。我们希望我们的设计策略对开发具有出色可靠性的荧光测温法具有重要意义。
更新日期:2017-10-26
down
wechat
bug