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Streamline-based purification of bacterial samples from liquefied sputum utilizing microfluidics
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-09-27 00:00:00 , DOI: 10.1039/c7lc00771j
Tian Wu 1, 2, 3, 4, 5 , Changjun Shao 6, 7, 8, 9, 10 , Lingjun Li 3, 4, 5, 11 , Shujing Wang 3, 4, 5, 11 , Qi Ouyang 1, 2, 3, 4, 5 , Yu Kang 6, 7, 8, 9, 10 , Chunxiong Luo 1, 2, 3, 4, 5
Affiliation  

The separation or purification of bacterial samples from a mixed cell suspension is critical in a variety of biomedical applications, such as sputum diagnostics and cell biology studies. We propose a streamline-based microfluidic filtration device for highly efficient purification of bacterial samples from a mixed cell suspension. The device is composed of tens of repeated streamline-based separation units that continuously filter the solution. By injecting a liquid sample such as liquefied human sputum solution through the device, approximately 50% of the injected sample solution can be collected from the filtration collection channels, which filter approximately 99.9% of the mammalian cells but retain approximately 60% to 90% of the bacteria. Different injection rates (0.2 ml h−1 to 30 ml h−1), different sample viscosities, and different initial bacterial densities were tested and confirmed that our separation method was robust. The easy operation, robustness and high efficiency indicate that our method may be useful for the separation or purification of bacterial samples from a mixed cell suspension, such as bacterial samples for sputum diagnostics.

中文翻译:

利用微流控技术从液化痰中以流水线为基础的细菌样品纯化

从混合细胞悬液中分离或纯化细菌样品在多种生物医学应用中至关重要,例如痰液诊断和细胞生物学研究。我们提出了一种基于流线的微流过滤设备,用于从混合细胞悬液中高效纯化细菌样品。该设备由数十个重复的基于流线的分离单元组成,可连续过滤溶液。通过将液体样本(例如液化人类痰液)通过该设备注入,可以从过滤收集通道中收集大约50%的注入样品溶液,该过滤收集通道可以过滤约99.9%的哺乳动物细胞,但保留约60%至90%的哺乳动物细胞。细菌。不同的注射速率(0.2 ml h -1至30 ml h -1),不同的样品粘度和不同的初始细菌密度进行了测试,并证实了我们的分离方法是可靠的。操作简便,坚固耐用且效率高,这表明我们的方法可用于从混合细胞悬液中分离或纯化细菌样品,例如用于痰液诊断的细菌样品。
更新日期:2017-10-26
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