The aim of this study is the development of a miniaturized voltammetric method for the determination of an antimycobacterial agent 1-hydroxy-N-(4-nitrophenyl)naphthalene-2-carboxamide (HNN) in a single drop (20 μL) of a solution by cathodic and anodic voltammetry at a glassy carbon electrode. Cyclic voltammetry was used to investigate its redox properties followed by the optimization of differential pulse voltammetric determination in a regular 10 mL volume. The optimal medium for the analytical application of both cathodic and anodic voltammetry was found to be Britton-Robinson buffer pH 7.0 and dimethyl sulfoxide (9 : 1, v/v). HNN gave one cathodic peak at around −0.6 V and one anodic peak at around +0.2 V vs. Ag|AgCl (3 mol L−1 KCl) reference electrode. Determination of HNN in a 10 mL volume gave the limit of quantification around 10 nmol L−1 by both adsorptive stripping anodic and cathodic voltammetry. Afterwards, miniaturized voltammetric methods in a single drop of solution (20 μL) were investigated. This approach requested some modifications of the cell design and voltammetric procedures. A novel method of removing dissolved oxygen in a single drop had to be developed and tested. Developed miniaturized voltammetric methods gave parameters comparable to the determination of HNN in 10 mL. The applicability of the miniaturized method was verified by the determination of HNN in a drop of a bacterial growth medium.

中文翻译：

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单滴溶液中新型抗分枝杆菌剂 1-羟基-N-(4-硝基苯基)萘-2-甲酰胺的伏安法

本研究的目的是开发一种微型伏安法，用于测定单滴 (20 μL) 溶液中的抗分枝杆菌剂 1-羟基-N-(4-硝基苯基)萘-2-甲酰胺 (HNN)通过阴极和阳极伏安法在玻碳电极上进行。循环伏安法用于研究其氧化还原特性，然后在常规 10 mL 体积中优化差分脉冲伏安法测定。发现阴极和阳极伏安法分析应用的最佳介质是 Britton-Robinson 缓冲液 pH 7.0 和二甲亚砜 (9:1, v/v)。HNN 在 -0.6 V 附近有一个阴极峰，在 +0.2 V 附近有一个阳极峰，相对于 Ag|AgCl (3 mol L-1 KCl) 参比电极。通过吸附剥离阳极和阴极伏安法测定 10 mL 体积中的 HNN 给出了约 10 nmol L-1 的定量限制。然后，研究了单滴溶液 (20 μL) 中的微型伏安法。这种方法要求对电池设计和伏安程序进行一些修改。必须开发和测试一种在单滴中去除溶解氧的新方法。开发的微型伏安法给出的参数可与 10 mL 中 HNN 的测定相媲美。通过测定一滴细菌生长培养基中的 HNN 验证了小型化方法的适用性。这种方法要求对电池设计和伏安程序进行一些修改。必须开发和测试一种在单滴中去除溶解氧的新方法。开发的微型伏安法提供的参数可与 10 mL 中 HNN 的测定相媲美。通过测定一滴细菌生长培养基中的 HNN 验证了小型化方法的适用性。这种方法要求对电池设计和伏安程序进行一些修改。必须开发和测试一种在单滴中去除溶解氧的新方法。开发的微型伏安法给出的参数与 10 mL 中 HNN 的测定相当。通过测定一滴细菌生长培养基中的 HNN 验证了小型化方法的适用性。