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High prevalence of hepatitis E virus in semen of infertile male and causes testis damage
Gut ( IF 24.5 ) Pub Date : 2017-08-31 , DOI: 10.1136/gutjnl-2017-314884
Fen Huang , Feiyan Long , Wenhai Yu , Jianwen Situ , Lijie Fu , Zhanlong He , Hao Dong , Chenchen Yang , Yunlong Li , Fan Yang , Daqiao Wei

We read with interest the paper by Todt et al 1 concerning the mutagenesis of Hepatitis E virus (HEV) caused by ribavirin treatment. HEV is a major cause of acute hepatitis worldwide and lacks a specific treatment.1 2 It replicates in many extrahepatic sites (spleen, kidney, brain and muscle3–5), but whether the testis is included is unknown. Active HEV has been detected in body fluids (blood,2 urine4 and milk6), but no report exists for semen. Thus, HEV prevalence in the semen of infertile males (n=185) sexually abstinent for at least 2 days was investigated by reverse transcriptase-PCR. Ethical approval was obtained from the Institutional Ethics Committee. Unexpectedly, 28.11% (52/185) of the males were HEV RNA positive (HEV RNA+; detectable HEV ORF1 and ORF2 were considered as HEV RNA+, figure 1A) with comparable viral titers to that from urine (see figure S1 in the online supplementary file 1). The prevalence of HEV in semen was higher in infertile males than in serum of pregnant women (1.82%) or the general population (0.58%) in the same city. ### Supplementary file 1 [SP1.pdf] Figure 1 (A) Positive rate of Hepatitis E virus (HEV) RNA in the semen of …

中文翻译:

不育男性精液中戊型肝炎病毒高流行并导致睾丸损伤

我们饶有兴趣地阅读了 Todt 等人 1 撰写的关于由利巴韦林治疗引起的戊型肝炎病毒 (HEV) 诱变的论文。HEV 是世界范围内急性肝炎的主要原因,并且缺乏特定的治疗方法。1 2 它在许多肝外部位(脾、肾、脑和肌肉 3-5)复制,但是否包括睾丸尚不清楚。已在体液(血液、2 尿液 4 和牛奶 6)中检测到活性 HEV,但尚无关于精液的报告。因此,通过逆转录酶-PCR 研究了禁欲至少 2 天的不育男性 (n=185) 精液中的 HEV 流行率。从机构伦理委员会获得伦理批准。出乎意料的是,28.11% (52/185) 的男性是 HEV RNA 阳性(HEV RNA+;可检测到的 HEV ORF1 和 ORF2 被认为是 HEV RNA+,图 1A) 的病毒滴度与尿液中的病毒滴度相当(参见在线补充文件 1 中的图 S1)。不孕男性精液中HEV的患病率高于孕妇血清(1.82%)或同城普通人群(0.58%)。### 补充文件 1 [SP1.pdf] 图 1 (A) 精液中戊型肝炎病毒 (HEV) RNA 的阳性率
更新日期:2017-08-31
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