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Mutations of Residues in Pocket P1 of a Cyclodipeptide Synthase Strongly Increase Product Formation
Journal of Natural Products ( IF 5.1 ) Pub Date : 2017-10-24 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00430 Kirsten Brockmeyer 1 , Shu-Ming Li 1
Journal of Natural Products ( IF 5.1 ) Pub Date : 2017-10-24 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00430 Kirsten Brockmeyer 1 , Shu-Ming Li 1
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Expression of a cyclodipeptide synthase gene from Nocardiopsis prasina (CDPS-Np) in Escherichia coli resulted in the formation of cyclo-(l-Tyr-l-Tyr) (1) as the minor and cyclo-(l-Tyr-l-Phe) (2) as the major products. Site-directed mutagenesis revealed a strong influence on product accumulation of the amino acid residues in pocket P1. An 8-fold increase in product formation for 1 and 10-fold for 2 were detected in the double mutant T82V_Y196F compared with the wild type.
中文翻译:
环二肽合酶口袋P1中的残基突变强烈增加了产物的形成
从cyclodipeptide合酶基因表达诺卡prasina(CDPS-Np个中)大肠杆菌导致的形成环- (升-Tyr-升-Tyr)(1)作为次要和环- (升-Tyr-升-Phe )(2)为主要产品。定点诱变揭示了对口袋P1中氨基酸残基产物积聚的强烈影响。一个8倍增加产物形成为1倍和10倍为2在双突变体T82V_Y196F检测与野生型进行比较。
更新日期:2017-10-24
中文翻译:
环二肽合酶口袋P1中的残基突变强烈增加了产物的形成
从cyclodipeptide合酶基因表达诺卡prasina(CDPS-Np个中)大肠杆菌导致的形成环- (升-Tyr-升-Tyr)(1)作为次要和环- (升-Tyr-升-Phe )(2)为主要产品。定点诱变揭示了对口袋P1中氨基酸残基产物积聚的强烈影响。一个8倍增加产物形成为1倍和10倍为2在双突变体T82V_Y196F检测与野生型进行比较。