Multiplicity in laccases among lignin degrading fungal species is of interest as it confers the ability to degrade several types of lignocellulosics. The combination of laccases produced on such substrates could be beneficial for treatment of complex aromatics, including dyes. In this study, we report on production of high units (679.6 U g⁻¹ substrate) of laccase on solid wheat bran (WB) by Cyathus bulleri. Laccase, purified from the culture filtrates of WB grown fungus, was effective for oxidation of veratryl alcohol, Reactive blue 21 and several dyes without assistance of externally added mediators. De novo sequencing of the ‘purified’ laccase lead to identification of several peptides that originated from different laccase genes. Transcriptome analysis of the fungus, cultivated on WB, confirmed presence of 8 isozymes, that were re-amplified and sequenced from the cDNA prepared from WB grown fungus. The 8 isozymes were grouped into 3 classes, based on their sequence relationship with other basidiomycete laccases. The isoforms produced on WB decolorized (by ∼57%) and degraded textile effluent far more effectively, compared to laccase obtained from Basal salt cultivated fungus. The decolorization and degradation was also accompanied by more than 95% reduction in phytotoxicity.

中文翻译：

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大麦仓鼠在小麦麸皮上产生的漆酶同工型的生物修复潜力的鉴定和评价

在降解木质素的真菌物种中漆酶的多样性是令人感兴趣的，因为它赋予了降解几种类型的木质纤维素的能力。在这样的底物上产生的漆酶的组合对于处理包括染料在内的复杂的芳族化合物可能是有益的。在这项研究中，我们报道了Cyathus bulleri在固态麦麸（WB）上生产高单位（679.6 U g ^-1底物）漆酶的情况。从WB生长的真菌的培养滤液中纯化的漆酶可有效氧化藜芦醇，活性蓝21和几种染料，而无需外部添加介体的帮助。从头开始“纯化的”漆酶的测序导致鉴定出源自不同漆酶基因的几种肽。在WB上培养的真菌进行转录组分析，确认存在8种同功酶，这些酶从WB生长的真菌制备的cDNA中进行了重新扩增和测序。根据8种同工酶与其他担子菌漆酶的序列关系，将其分为3类。与从基础盐栽培真菌获得的漆酶相比，在WB上产生的同工型脱色（约57％）并能更有效地降解纺织品废水。脱色和降解还伴随着超过95％的植物毒性降低。