Selenocysteine (Sec) is known as the 21st amino acid that is incorporated into selenoproteins (SePs) and plays vital roles in living systems. Accordingly, development of fluorescent detection method for Sec is of significance. Herein, a readily available fluorescent Sec probe is reported. Although this probe has been reported before for detection of biothiols, the studies in this work show that it can be used for rapid, high selective and sensitive detection of Sec over biothiols and many other analytes with dual colorimetric and fluorescent turn-on signal changes simply by slightly changing the detection conditions. The detection limit of this probe for Sec was found to be about 47 nM with a wide linear range (0–80 μM). In addition, this probe has low cytotoxicity and is able to detect Sec in serum samples and in living cells, indicating it can be used as a potential new molecular tool for Sec detection.

硒代半胱氨酸（Sec）被称为硒酸（SePs）中的第21个氨基酸，在生命系统中起着至关重要的作用。因此，开发用于Sec的荧光检测方法具有重要意义。在此，报道了一种容易获得的荧光Sec探针。尽管以前已经报道过该探针可用于检测生物硫醇，但这项工作的研究表明，它可用于比生物硫醇和许多其他具有双色度和荧光开启信号变化的分析物更快速，高选择性和灵敏的Sec检测通过稍微改变检测条件。发现该探针对Sec的检出限约为47 nM，线性范围很广（0–80μM）。此外，该探针的细胞毒性低，能够检测血清样品和活细胞中的Sec，