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Nuclear and Fluorescent Labeled PD-1-Liposome-DOX-64Cu/IRDye800CW Allows Improved Breast Tumor Targeted Imaging and Therapy
Molecular Pharmaceutics ( IF 4.9 ) Pub Date : 2017-10-19 00:00:00 , DOI: 10.1021/acs.molpharmaceut.7b00649
Yang Du 1 , Xiaolong Liang 2 , Yuan Li 1, 3 , Ting Sun 1, 3 , Zhengyu Jin 3 , Huadan Xue 3 , Jie Tian 1
Affiliation  

The overexpression of programmed cell death-1 (PD-1) in tumors as breast cancer makes it a possible target for cancer imaging and therapy. Advances in molecular imaging, including radionuclide imaging and near-infrared fluorescence (NIRF) imaging, enable the detection of tumors with high sensitivity. In this study, we aim to develop a novel PD-1 antibody targeted positron emission tomography (PET) and NIRF labeled liposome loaded with doxorubicin (DOX) and evaluate its application for in vivo cancer imaging and therapy. IRDye800CW and 64Cu were conjugated to liposomes with PD-1 antibody labeling, and DOX was inside the liposomes to form theranostic nanoparticles. The 4T1 tumors were successfully visualized with PD-1-Liposome-DOX-64Cu/IRDye800CW using NIRF/PET imaging. The bioluminescent imaging (BLI) results showed that tumor growth was significantly inhibited in the PD-1-Liposome-DOX-treated group than the IgG control. Our results highlight the potential of using dual-labeled theranostic PD-1 mAb-targeted Liposome-DOX-64Cu/IRDye800CW for the management of breast tumor.

中文翻译:

核和荧光标记的PD-1-Liposome-DOX- 64 Cu / IRDye800CW可以改善乳腺肿瘤靶向成像和治疗

作为乳腺癌,在肿瘤中过度表达程序性细胞死亡1(PD-1)使其成为癌症成像和治疗的可能靶标。分子成像技术的进步,包括放射性核素成像和近红外荧光(NIRF)成像,使得能够以高灵敏度检测肿瘤。在这项研究中,我们旨在开发一种新型的PD-1抗体靶向正电子发射断层扫描(PET)和载有阿霉素(DOX)的NIRF标记脂质体,并评估其在体内癌症成像和治疗中的应用。用PD-1抗体标记将IRDye800CW和64 Cu偶联到脂质体上,DOX在脂质体内,形成治疗性纳米颗粒。用PD-1-Liposome-DOX- 64成功可视化了4T1肿瘤使用NIRF / PET成像的Cu / IRDye800CW。生物发光成像(BLI)结果表明,与IgG对照相比,PD-1-脂质体-DOX处理组的肿瘤生长受到明显抑制。我们的结果凸显了使用双重标记的靶向治疗性PD-1 mAb的脂质体-DOX- 64 Cu / IRDye800CW进行乳腺肿瘤治疗的潜力。
更新日期:2017-10-19
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