We have constructed an aptamer immobilized gold atomic cluster mediated, ultrasensitive electrochemical biosensor (Apt/AuAC/Au) for LPS detection without any additional signal amplification strategy. The aptamer self-assemble onto the gold atomic clusters makes Apt/AuAC/Au an excellent platform for the LPS detection. Differential pulse voltammetry and EIS were used for the quantitative LPS detection. The Apt/AuAC/Au sensor offers an ultrasensitive and selective detection of LPS down to 7.94 × 10^–21 M level with a wide dynamic range from 0.01 attomolar to 1 pM. The sensor exhibited excellent selectivity and stability. The real sample analysis was performed by spiking the diluted insulin sample with various concentration of LPS and obtained recovery within 2% error value. The sensor is found to be more sensitive than most of the literature reports. The simple and easy way of construction of this sensor provides an efficient and promising detection of an even trace amount of LPS.

我们已经构建了适配子固定的金原子簇介导的超灵敏电化学生物传感器（Apt / AuAC / Au），用于LPS检测，无需任何其他信号放大策略。适体自组装到金原子簇上，使Apt / AuAC / Au成为LPS检测的出色平台。差分脉冲伏安法和EIS用于定量LPS检测。Apt / AuAC / Au传感器可对LPS进行超灵敏的选择性检测，检测范围低至7.94×10 ^–21 M水平具有0.01至1 pM的宽动态范围。该传感器具有出色的选择性和稳定性。通过用各种浓度的LPS加标稀释的胰岛素样品进行真实样品分析，并在2％误差值内获得回收率。发现该传感器比大多数文献报道更敏感。这种传感器的简单构造方式可以有效，有希望地检测到甚至痕量的LPS。