Wash-free fluorescence imaging process has intrinsic nature of monitoring biochemical process in real time fashion and enable us to get more insight on living organism. In this contribution, it was proved that boronic acid derived salicylidenehydrazone complexes (BSs) are suitable candidates for wash-free fluorescence labels. Through pre-installation of organelles' targeting group morpholine and triphenylphosphonium (TPP⁺) on phenyboronic acid and then assembly it with salicylidenehydrazone ligand by dehydration and dative N-B bond, Lyso-BS and Mito-BS were acquired, which were shown to selectively stain lysosomes and mitochondria with circumventing tedious wash-off procedures in Hela cells respectively, without inducing any appreciable cytotoxicity. Our findings indicate that BSs are potential candidates as wash-free fluorescent stains.

免洗荧光成像过程具有实时监控生化过程的内在本质，使我们能够对生物体有更多的了解。在这一贡献中，证明了硼酸衍生的水杨基hydr复合物（BSs）是免洗荧光标记的合适候选物。通过预先安装细胞器的靶向组吗啉和三苯基phosph（TPP ⁺）在苯硼酸上，然后通过脱水和顺式NB键将其与水杨叉hydr配体组装在一起，获得了Lyso-BS和Mito-BS，它们显示出分别通过绕过Hela细胞的繁琐冲洗程序选择性地对溶酶体和线粒体进行了染色，而无需诱导任何明显的细胞毒性。我们的发现表明，BSs是免洗荧光染料的潜在候选者。