AbstractThe authors describe two kinds of immunochromatographic assays. The first is based on the use of dyed polymer microspheres (MICA), the another on the use of quantum dots (QICA). Both enable visual detection of enrofloxacin (ENR) in animal tissue and milk. Both the MICA and the QICA have visual limits of detection of 1 μg·L−1 when working in buffer, of 5 μg·kg−1 in case of animal tissue, and of 10 μg·L−1 in case of milk. Other quinolones do not interfere. The MICA and QICA described here are convenient and fairly rapid in that the detection process (including sample pretreatment and assay) takes 20 min only which is far less than the commercial enzyme-linked immunosorbent assay (ELISA) test kit which requires 120 min. The MICA and QICA are more sensitive than the immunochromatographic assay using colloidal gold labels and the same polyclonal antibody. The results of analysis of spiked samples via MICA and QICA are in good agreement with those obtained by the commercial ELISA test kit. Graphical abstractA visual dyed polymer microsphere-based immunochromatographic assay (MICA) and a visual quantum-dot-based immunochromatographic assay (QICA) are described for the rapid, specific, qualitative, semi-quantitative, and on-site detection of enrofloxacin residues in animal origin foods.

中文翻译：

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使用染色聚合物微球和量子点对恩诺沙星进行可视化和快速侧流免疫层析测定

摘要作者描述了两种免疫层析分析。第一种是基于染色聚合物微球（MICA）的使用，另一种是基于量子点（QICA）的使用。两者都可以对动物组织和牛奶中的恩诺沙星 (ENR) 进行目视检测。MICA 和 QICA 在缓冲液中工作时的视觉检测限为 1 μg·L-1，在动物组织中为 5 μg·kg-1，在牛奶中为 10 μg·L-1。其他喹诺酮类药物不干扰。这里描述的 MICA 和 QICA 方便且相当快速，因为检测过程（包括样品预处理和测定）只需要 20 分钟，远少于需要 120 分钟的商业酶联免疫吸附测定 (ELISA) 检测试剂盒。MICA 和 QICA 比使用胶体金标记和相同多克隆抗体的免疫层析测定更灵敏。通过 MICA 和 QICA 分析加标样品的结果与商业 ELISA 检测试剂盒获得的结果非常一致。图形摘要描述了一种基于视觉染色聚合物微球的免疫色谱分析 (MICA) 和一种基于视觉量子点的免疫色谱分析 (QICA)，用于快速、特异性、定性、半定量和现场检测动物中的恩诺沙星残留原产地食品。