AbstractThe authors describe a microreactor for performing a multiplexed polymerase chain reaction (PCR) which is operated with minimal accessories such as a single heater for gene amplification and a hand-held syringe for sample actuation. It was fabricated by wrapping a polytetrafluoroethylene (PTFE) tube around a rectangular poly(dimethylsiloxane) (PDMS) block having a predetermined thickness. The resulting portable microreactor was applied to PCR using a single heater because the rectangular PDMS block spatially segregated the upper and lower arrays of the PTFE tube. This warrants the adjustment of distinct temperatures inside the upper and lower tubes. A hand-held plastic syringe was connected to the inlet of the PTFE tube and used as a portable pump to achieve a homogeneous transport of a sample liquid inside the microreactor. The use of gas-impermeable PTFE prevents the formation of bubbles during thermal cycling. The microreactor was successfully applied to the amplification of typical DNA fragments of three foodborne pathogens in less than 30 min. In our perception, this method paves the way to the construction of a truly portable PCR chip that is applicable to rapid clinical diagnosis and the detection of foodborne pathogens. Graphical abstractᅟ

中文翻译：

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聚合酶链反应所需附件最少的便携式微反应器：在食源性病原体测定中的应用

摘要作者描述了一种用于执行多重聚合酶链反应 (PCR) 的微反应器，该反应器使用最少的附件进行操作，例如用于基因扩增的单个加热器和用于样本驱动的手持注射器。它是通过将聚四氟乙烯 (PTFE) 管包裹在具有预定厚度的矩形聚（二甲基硅氧烷）（PDMS）块上来制造的。由于矩形 PDMS 块在空间上隔离了 PTFE 管的上部和下部阵列，因此使用单个加热器将所得的便携式微反应器应用于 PCR。这保证了上下管内不同温度的调整。手持式塑料注射器连接到 PTFE 管的入口，用作便携式泵，以实现微反应器内样品液体的均匀传输。使用不透气的 PTFE 可防止在热循环过程中形成气泡。该微反应器在不到30分钟的时间内成功应用于三种食源性病原体典型DNA片段的扩增。在我们看来，该方法为构建真正适用于临床快速诊断和食源性病原体检测的便携式 PCR 芯片铺平了道路。图形摘要ᅟ