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Comprehensive evaluation of the cytotoxicity of CdSe/ZnS quantum dots in Phanerochaete chrysosporium by cellular uptake and oxidative stress
Environmental Science: Nano ( IF 7.3 ) Pub Date : 2017-09-04 00:00:00 , DOI: 10.1039/c7en00517b
Liang Hu 1, 2, 3, 4, 5 , Jia Wan 1, 2, 3, 4, 5 , Guangming Zeng 1, 2, 3, 4, 5 , Anwei Chen 3, 4, 6, 7 , Guiqiu Chen 1, 2, 3, 4, 5 , Zhenzhen Huang 1, 2, 3, 4, 5 , Kai He 1, 2, 3, 4, 5 , Min Cheng 1, 2, 3, 4, 5 , Chengyun Zhou 1, 2, 3, 4, 5 , Weiping Xiong 1, 2, 3, 4, 5 , Cui Lai 1, 2, 3, 4, 5 , Piao Xu 1, 2, 3, 4, 5
Affiliation  

The growing potential of quantum dots (QDs) in biological and biomedical applications has raised considerable concern due to their toxicological impact. Consequently, it is urgent to elucidate the underlying toxicity mechanism of QDs. In this work, we comprehensively investigated the cellular uptake of four CdSe/ZnS QDs (COOH CdSe/ZnS 525, COOH CdSe/ZnS 625, NH2 CdSe/ZnS 525, and NH2 CdSe/ZnS 625) and induced physiological responses in Phanerochaete chrysosporium (P. chrysosporium) through inductively coupled plasma optical emission spectroscopy, confocal laser scanning microscopy, and the determination of malondialdehyde content, superoxide level, superoxide dismutase activity, catalase activity and glutathione level. The results showed that the four CdSe/ZnS QDs accumulated largely in the hyphae and caused oxidative stress to P. chrysosporium in the tested concentration range (10–80 nM). Furthermore, the cellular uptake and cytotoxicity were related to the physicochemical properties of the QDs, such as particle size and surface charges. Negatively charged CdSe/ZnS QDs with small size could be more easily ingested by P. chrysosporium than large ones; thus small size CdSe/ZnS QDs were more cytotoxic to P. chrysosporium. On the other hand, small negatively charged CdSe/ZnS QDs resulted in greater cytotoxicity than large negatively charged CdSe/ZnS QDs. The obtained results offer valuable information for revealing the toxicity mechanism of QDs in living cells.

中文翻译:

通过细胞摄取和氧化应激综合评价金缕藻中CdSe / ZnS量子点的细胞毒性

量子点(QDs)在生物和生物医学应用中的增长潜力由于其毒理学影响而引起了人们的极大关注。因此,迫切需要阐明量子点的潜在毒性机制。在这项工作中,我们全面研究了四种CdSe / ZnS QDs(COOH CdSe / ZnS 525,COOH CdSe / ZnS 625,NH 2 CdSe / ZnS 525和NH 2 CdSe / ZnS 625)的细胞吸收以及在Phanerochaete中诱导的生理反应。金孢子P. chrysosporium)通过电感耦合等离子体发射光谱法,共聚焦激光扫描显微镜检查以及丙二醛含量,超氧化物水平,超氧化物歧化酶活性,过氧化氢酶活性和谷胱甘肽水平的测定。结果表明,在所测试的浓度范围(10-80 nM)内,四个CdSe / ZnS QDs大量积累在菌丝中,并导致对金黄色葡萄球菌的氧化应激。此外,细胞摄取和细胞毒性与量子点的物理化学性质有关,例如粒径和表面电荷。具有负电荷的小尺寸CdSe / ZnS量子点比大孢子更容易被金黄色葡萄球菌摄取。因此,小尺寸的CdSe / ZnS QD对金黄色葡萄球菌更具细胞毒性。另一方面,小的带负电荷的CdSe / ZnS量子点比大带负电荷的CdSe / ZnS量子点具有更大的细胞毒性。获得的结果为揭示量子点在活细胞中的毒性机理提供了有价值的信息。
更新日期:2017-10-13
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