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Ecotoxicological assessment of nanoparticle-containing acrylic copolymer dispersions in fairy shrimp and zebrafish embryos
Environmental Science: Nano ( IF 7.3 ) Pub Date : 2017-08-22 00:00:00 , DOI: 10.1039/c7en00385d
Tamara S. Galloway 1, 2, 3 , Yuktee Dogra 1, 2, 3 , Natalie Garrett 2, 3, 4 , Darren Rowe 1, 2, 3 , Charles R. Tyler 1, 2, 3 , Julian Moger 2, 3, 4 , Eva Lammer 5, 6, 7 , Robert Landsiedel 8, 9, 10 , Ursula G. Sauer 10, 11, 12 , Gertrud Scherer 9, 10, 13 , Wendel Wohlleben 9, 10, 14 , Karin Wiench 10, 15, 16
Affiliation  

Nanoparticle-containing polymer dispersions are widely used, but little is known of their environmental effects. We studied the bioavailability, uptake, tissue localisation and effects of nanoparticle-containing acrylic copolymer (ACP) dispersions (mean nanoparticle sizes: 80 nm and 110 nm) in aquatic invertebrates (Thamnocephalus platyurus; fairy shrimp) and Danio rerio zebrafish embryos after aquatic exposures. Dietary exposure tests were enabled using Casper zebrafish that lack skin pigmentation allowing for bio-imaging of uptake and internal distribution. Aqueous exposures of 1000 and 2500 mg L−1 80 nm-ACP or 110 nm-ACP showed no acute toxicity in fairy shrimp or zebrafish, constituting a non-toxic classification according to the United Nations Globally Harmonised System of Classification and Labelling of Chemicals threshold (100 mg L−1). Similarly, dietary exposures resulted in no ecotoxicological effects. In Casper zebrafish fed with 80 nm-ACP-spiked food, hyperspectral signals derived using coherent Raman scattering (CRS) indicated that test material was present in the intestine, and possibly in the liver, but not in other organs. CRS imaging indicated that the chemical composition of the yolk sac of an 80 nm-ACP exposed zebrafish (aquatic exposure) was altered, attributed to a change in lipid metabolism, although we could not confirm with certainty that the test material was physically present in the yolk sac. These results illustrate how CRS microscopy can be used to investigate the bioaccumulation of organic nanomaterials, provided that they induce hyperspectral profiles distinct from the biological samples. In conclusion, both 80 nm- and 110 nm-ACP dispersions are internalised through dietary exposure, but are not associated with significant toxic effects.

中文翻译:

仙虾和斑马鱼胚胎中含纳米颗粒的丙烯酸共聚物分散体的生态毒理学评估

含纳米颗粒的聚合物分散体被广泛使用,但对其环境影响知之甚少。我们研究了在水生无脊椎动物(Thamnocephalus platyurus;仙女虾)和Danio rerio斑马鱼胚胎在水接触后,含纳米颗粒的丙烯酸共聚物(ACP)分散体(平均纳米颗粒大小:80 nm和110 nm)的生物利用度,吸收,组织定位和影响。。使用缺少皮肤色素的Casper斑马鱼可以进行饮食暴露测试,从而可以对摄取和内部分布进行生物成像。1000和2500 mg L -1的水暴露80 nm-ACP或110 nm-ACP在仙虾或斑马鱼中没有显示出急性毒性,根据联合国全球化学品统一分类和标签制度阈值(100 mg L -1)构成无毒分类。同样,饮食接触也没有生态毒理作用。在卡斯珀斑马鱼喂食了80 nm-ACP的食物,使用相干拉曼散射(CRS)得出的高光谱信号表明测试物质存在于肠中,可能存在于肝脏中,但在其他器官中不存在。CRS成像表明,暴露于80 nm-ACP的斑马鱼的卵黄囊的化学成分(水生暴露)发生了变化,这归因于脂类代谢的变化,尽管我们不能确定地确定受试物质是否存在于动物体内。卵黄囊。这些结果说明了CRS显微镜技术可用于研究有机纳米材料的生物蓄积性,前提是它们可诱导出不同于生物样品的高光谱图。总之,无论是80 nm还是110 nm的ACP分散体都是通过饮食暴露而被内在化的,
更新日期:2017-10-13
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