An inexpensive stability−indicating anodic voltammetric method for rapid determination of two non-classical β-lactam antibiotics; Meropenem (MP) and Ertapenem (EP) has been developed and validated. The method was based on the enhancement of voltammetric response at a disposable graphite pencil electrode (GPE). Differential pulse voltammetric (DPV) method was developed for quantification of both drugs in B−R buffer solution (pH 2.0) at GPE. The GPE displayed very good voltammetric behavior with significant enhancement of the peak current compared to glassy carbon electrode (GCE). Stress stability studies were performed using 0.5 M of either HCl or NaOH and H2O2. Mass and infrared spectroscopy were used for identification of degradants and their pathways were illustrated. Under optimal conditions, the peak currents showed a linear dependence with drug concentrations. The achieved limits of detection (LOD) were 1.23, 2.07 and 1.50 μM for MP and two waves of EP, respectively. The developed voltammetric method was successfully applied for direct determination of MP and EP in drug substances, pharmaceutical vials and in presence of either their corresponding hydrolytic, oxidative-degradants or interfering substances with no potential interferences. The differential pulse voltammograms were highly advantageous and applicable in QC laboratories for rapid, selective micro-determination of MP and EP.

中文翻译：

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快速选择性测定存在不同降解物和干扰物质的两种非经典 β-内酰胺的廉价电化学传感器的挑战方法

一种廉价的稳定性指示阳极伏安法，用于快速测定两种非经典 β-内酰胺抗生素；美罗培南 (MP) 和厄他培南 (EP) 已经开发和验证。该方法基于增强一次性石墨铅笔电极 (GPE) 的伏安响应。差分脉冲伏安法 (DPV) 被开发用于在 GPE 的 B-R 缓冲溶液 (pH 2.0) 中对两种药物进行定量。与玻璃碳电极 (GCE) 相比，GPE 显示出非常好的伏安行为，峰值电流显着增强。使用 0.5 M HCl 或 NaOH 和 H2O2 进行应力稳定性研究。质谱和红外光谱用于鉴定降解物并说明其途径。在最佳条件下，峰值电流与药物浓度呈线性相关。MP 和两波 EP 的检测限 (LOD) 分别为 1.23、2.07 和 1.50 μM。所开发的伏安法已成功应用于直接测定原料药、药瓶中的 MP 和 EP，以及在它们相应的水解、氧化降解物或干扰物质存在的情况下，没有潜在的干扰。差分脉冲伏安图在 QC 实验室中非常有利，适用于 MP 和 EP 的快速、选择性微量测定。药瓶，并存在相应的水解、氧化降解物或无潜在干扰的干扰物质。差分脉冲伏安图在 QC 实验室中非常有利，适用于 MP 和 EP 的快速、选择性微量测定。药瓶，并存在相应的水解、氧化降解物或无潜在干扰的干扰物质。差分脉冲伏安图在 QC 实验室中非常有利，适用于 MP 和 EP 的快速、选择性微量测定。