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Preservation of Cell Structure, Metabolism, and Biotransformation Activity of Liver‐On‐Chip Organ Models by Hypothermic Storage
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-09-27 , DOI: 10.1002/adhm.201700616
Marko Gröger 1 , Julia Dinger 2 , Michael Kiehntopf 3 , Frank T. Peters 2 , Ursula Rauen 4 , Alexander S. Mosig 1
Affiliation  

The liver is a central organ in the metabolization of nutrition, endogenous and exogenous substances, and xenobiotic drugs. The emerging organ‐on‐chip technology has paved the way to model essential liver functions as well as certain aspects of liver disease in vitro in liver‐on‐chip models. However, a broader use of this technology in biomedical research is limited by a lack of protocols that enable the short‐term preservation of preassembled liver‐on‐chip models for stocking or delivery to researchers outside the bioengineering community. For the first time, this study tested the ability of hypothermic storage of liver‐on‐chip models to preserve cell viability, tissue morphology, metabolism and biotransformation activity. In a systematic study with different preservation solutions, liver‐on‐chip function can be preserved for up to 2 d using a derivative of the tissue preservation solution TiProtec, containing high chloride ion concentrations and the iron chelators LK614 and deferoxamine, supplemented with polyethylene glycol (PEG). Hypothermic storage in this solution represents a promising method to preserve liver‐on‐chip function for at least 2 d and allows an easier access to liver‐on‐chip technology and its versatile and flexible use in biomedical research.

中文翻译:

通过低温保存来保存肝癌器官模型的细胞结构,代谢和生物转化活性

肝脏是营养,内源性和外源性物质以及异种药物代谢的中心器官。新兴的单芯片器官技术为在单芯片肝脏模型中体外模拟基本肝功能以及某些肝脏疾病的铺平了道路。但是,由于缺乏能够短期保存预先组装的肝片模型以供储存或交付给生物工程界以外的研究人员的协议,该技术在生物医学研究中的广泛应用受到限制。这项研究首次测试了肝片模型的低温保存能力,以保持细胞活力,组织形态,代谢和生物转化活性。在一项针对不同保存解决方案的系统研究中,使用组织保存溶液TiProtec的衍生物可以保留最多2 d的肝功能,该溶液包含高氯离子浓度以及铁螯合剂LK614和去铁胺,并辅以聚乙二醇(PEG)。该解决方案中的低温储存是一种有希望的方法,可以将肝片功能至少保留2 d,并且可以更轻松地使用肝片技术及其在生物医学研究中的多功能性和灵活性。
更新日期:2017-09-27
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