2,3-Butanediol (2,3-BD), especially when it exists in the chirally pure form, is a valuable chemical feedstock with numerous applications in various industries. However, in most cases, the 2,3-BD naturally produced by the microorganism is a mixture of two of the three isomers (d-2,3-BD, l-2,3-BD, and meso-2,3-BD), which has restricted applications and thus a much lower value than the chirally pure one. In this study, we report a new isolate Bacillus spp. FJ-4 strain, which can produce highly chirally pure d-2,3-BD (>99.9%) to high levels, with very high productivity and yield. The composition of the fermentation medium for the strain was sequentially optimized using statistical experimental methodologies. During a fed-batch experiment, 100.0 g/L of d-2,3-BD was produced from 226.8 g/L glucose, with a yield of 0.44 g/g (88.2% of the theoretical maximum). Acetoin was the primary byproduct, and no other byproduct such as lactate, acetate, or ethanol was detected, which would be advantageous for the downstream purification process. Moreover, no special conditions such as limited oxygen level (which generally limits the cell growth and thus final d-2,3-BD production) are required for the fermentation. These results indicated that Bacillus spp. FJ-4 is a robust workhorse for efficient d-2,3-BD production from low-value carbon sources.

中文翻译：

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用新分离的芽孢杆菌菌株生产高水平的手性纯d-2,3-丁二醇

2,3-丁二醇（2,3-BD），特别是当它以手性纯净形式存在时，是一种有价值的化学原料，在各种行业中都有广泛的应用。但是，在大多数情况下，微生物自然产生的2,3-BD是三种异构体中的两种的混合物（d -2,3-BD，l -2,3-BD和内消旋-2,3- BD），它的应用受到限制，因此其值比手性纯正的低得多。在这项研究中，我们报告了一种新的分离芽孢杆菌。FJ-4菌株，可产生高度手性纯净的d-2,3-BD（> 99.9％）达到高水平，具有很高的生产率和产量。使用统计学实验方法顺序优化菌株的发酵培养基的组成。在分批补料实验中，由226.8 g / L葡萄糖产生100.0 g / L d -2,3-BD，产量为0.44 g / g（理论最大值的88.2％）。丙酮是主要的副产物，未检测到其他副产物，如乳酸盐，乙酸盐或乙醇，这对于下游纯化过程将是有利的。而且，发酵不需要特殊条件，例如有限的氧水平（其通常限制细胞生长并因此限制最终的d -2，3-BD产生）。这些结果表明芽孢杆菌属。FJ-4是从低碳来源有效生产d -2,3-BD的强大工具。