当前位置: X-MOL 学术Analyst › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(II) complexes for heparin detection
Analyst ( IF 4.2 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1039/c7an01253e
Jing Li 1, 2, 3, 4, 5 , Meng Cheng 1, 2, 3, 4, 5 , Mei-Jin Li 1, 2, 3, 4, 5
Affiliation  

A sensitive assay was demonstrated for the colorimetric and luminescent detection of heparin. Positively charged [Ru(phen)2np]2+ (np: naphtho[2,3-f][1,10]phenanthroline) can interact with negatively charged AuNPs through electrostatic interaction, leading to the luminescence quenching of [Ru(phen)2np]2+via energy transfer as well as the aggregation of AuNPs together with a color change in solution from red to blue. Upon the addition of negatively charged heparin to gold nanoparticles functionalized by ruthenium(II) complexes, the emission of [Ru(phen)2np]2+ recovered due to the competitive adsorption of [Ru(phen)2np]2+ to heparin and AuNPs. A linear calibration curve was obtained in the 0.4 μM to 100 μM range and 0.1 μM to 6 μM with a low detection limit of 0.22 μM and 0.024 μM based on 3σ/s for heparin quantification in buffer solution and in 1% fetal bovine serum (FBS), respectively. Interestingly, the naked eye alone can judge the presence of 0.02 μM heparin without the aid of any advanced instruments by color change. Thus a convenient and visual method to detect heparin was established.

中文翻译:

一种基于钌(II)配合物对金纳米粒子功能化的发光和比色探针,用于肝素检测

肝素的比色和发光检测证明了一种灵敏的检测方法。带正电的[Ru(phen)2 np] 2+(np:萘并[2,3- f ] [1,10]菲咯啉)可通过静电相互作用与带负电的AuNP相互作用,从而导致[Ru(phen)2的发光猝灭)2 np] 2+通过能量转移以及AuNP的聚集以及溶液从红色到蓝色的颜色变化。在将带负电荷的肝素添加到被钌(II)络合物官能化的金纳米颗粒上时,由于[Ru(phen)的竞争性吸附]而恢复了[Ru(phen)2 np] 2+的发射。2 np] 2+对肝素和AuNPs。线性校正曲线的取值范围为0.4μM至100μM和0.1μM至6μM,基于 / s的低检测限,为0.22μM和0.024μM,用于缓冲溶液和1%胎牛血清中肝素的定量(FBS)。有趣的是,仅靠肉眼就可以判断0.02μM肝素的存在,而无需借助任何先进的仪器来改变颜色。因此,建立了一种方便,直观的肝素检测方法。
更新日期:2017-09-25
down
wechat
bug