All together now: Chemical protein synthesis enabled the generation of homogeneous atypical ubiquitin (Ub) chains that are difficult to prepare by other approaches. A ligation method mediated by an isopeptide-linked Ub isomer was developed to assemble the Ub chains. In this approach, a premade isopeptide-linked 76-mer (isoUb) bearing an N-terminal Cys and a C-terminal hydrazide is the key building block.

中文翻译：

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通过使用异肽连接的Ub异构体的非典型泛素链的实用化学合成。

现在在一起：化学蛋白质合成可以生成均质的非典型遍在蛋白（Ub）链，而这是其他方法难以制备的。开发了由异肽连接的Ub异构体介导的连接方法以组装Ub链。在这种方法中，预制的异肽连接的76-mer（isoUb）带有N端Cys和C端酰肼是关键的组成部分。