Helicobacter pylori α1–3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L⁻¹ culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).

中文翻译：

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幽门螺杆菌α1–3 / 4岩藻糖基转移酶（Hp3 / 4FT）催化的一锅多酶（OPME）合成Lewis抗原和人乳岩藻糖苷

幽门螺杆菌α1-3/ 4-岩藻糖基转移酶（Hp3 / 4FT）在大肠杆菌中以30 mg L ^-1的培养水平表达，并在单罐多酶（OPME）系统中用作多种催化剂以实现高产量含L-岩藻糖的碳水化合物，包括Lewis抗原，例如Lewis a，b和x，O-硫酸化的Lewis x和唾液酸化的Lewis x，以及人乳岩藻糖苷，例如3-岩藻糖半乳糖（3-FL），乳酸-N-岩藻多糖（LNFP）III和Lacto- N-二fuco-己糖（LNDFH）II和III。值得注意的是，虽然四糖的difucosylation使用供体的过量量容易地实现，LNFP III的合成通过的Hp3的/ 4FT催化选择性岩藻糖基实现Ñ -acetyllactosamine在lacto-（的LacNAc）成分Ñ -neotetraose（的LNnT）。