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In vivo probing of nascent RNA structures reveals principles of cotranscriptional folding
Nucleic Acids Research ( IF 14.9 ) Pub Date : 2017-07-14 , DOI: 10.1093/nar/gkx617
Danny Incarnato 1, 2 , Edoardo Morandi 1, 2 , Francesca Anselmi 1, 2 , Lisa M. Simon 1, 2 , Giulia Basile 1, 2 , Salvatore Oliviero 1, 2
Affiliation  

Defining the in vivo folding pathway of cellular RNAs is essential to understand how they reach their final native conformation. We here introduce a novel method, named Structural Probing of Elongating Transcripts (SPET-seq), that permits single-base resolution analysis of transcription intermediates’ secondary structures on a transcriptome-wide scale, enabling base-resolution analysis of the RNA folding events. Our results suggest that cotranscriptional RNA folding in vivo is a mixture of cooperative folding events, in which local RNA secondary structure elements are formed as they get transcribed, and non-cooperative events, in which 5′-halves of long-range helices get sequestered into transient non-native interactions until their 3′ counterparts have been transcribed. Together our work provides the first transcriptome-scale overview of RNA cotranscriptional folding in a living organism.

中文翻译:

体内探测新生RNA结构揭示了共转录折叠的原理

定义细胞RNA的体内折叠途径对于了解它们如何达到其最终天然构象至关重要。我们在这里介绍一种新颖的方法,称为延伸转录本的结构探测(SPET-seq),该方法可以在转录组范围内对转录中间体的二级结构进行单碱基分辨率分析,从而可以对RNA折叠事件进行碱基分辨率分析。我们的结果表明,共转录RNA在体内可折叠是合作折叠事件(其中转录时形成局部RNA二级结构元件)和非合作事件(其中5'-远程螺旋的5'部分被隔离成短暂的非天然相互作用)的混合物, '对应物已被转录。我们的工作共同提供了在活生物体中RNA共转录折叠的第一个转录组规模的概述。
更新日期:2017-09-21
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