Gegenqinlian decoction (GQLD) is one of the classic traditional Chinese medicine. The formula consists of four herbal medicines including Radix Puerariae lobatae, Radix Scutellariae, Coptidis Rhizoma, and Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle. In this study, an ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry method was employed to investigate the major constituents in GQLD. Liquid chromatography separation was conducted on a HSS T3 column (2.1 mm×100 mm, 1.8 μm) with mobile phase consisting of acetonitrile and 0.1% formic acid-water at the flow rate of 0.30 mL/min. Accurate mass measurement for molecular ions (below 2 ppm in most cases) and characteristic fragment ions could represent reliable identification criteria for these compounds. In total, 134 compounds in GQLD were identified or tentatively characterized mainly including flavonoids, alkaloids and triterpenoids. In conclusion, the UHPLC-FT-ICR-MS is a highly efficient technique to separate and identify constituents in complex matrices of traditional Chinese medicine. These results could provide a basis for quality control and further in vivo research of GQLD.

中文翻译：

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超高效液相色谱-傅里叶变换离子回旋共振质谱法检测葛根gen连汤中的化学成分

葛根勤联汤（GQLD）是经典的中药之一。该配方由四种草药组成，包括葛根，黄S，黄连和甘草等。本研究采用超高效液相色谱法结合傅里叶变换离子回旋共振质谱法研究GQLD中的主要成分。在HSS T3色谱柱（2.1 mm×100 mm，1.8μm）上进行液相色谱分离，流动相由乙腈和0.1％甲酸-水组成，流速为0.30 mL / min。分子离子（大多数情况下低于2 ppm）的精确质量测量和特征性碎片离子可以代表这些化合物的可靠鉴定标准。总共，鉴定或初步鉴定了GQLD中的134种化合物，主要包括类黄酮，生物碱和三萜类化合物。总之，UHPLC-FT-ICR-MS是分离和鉴定中药复杂基质中成分的高效技术。这些结果可为质量控制和进一步的GQLD体内研究提供基础。