Herein, we develop a simple fluorescence method for the sensitive detection of microRNAs based on duplex specific nuclease-assisted target recycling and pyrene excimer switching. This method is extremely sensitive with a detection limit of 0.58 fM, and it can be applied to absolutely detect endogenous microRNAs in cancer cells, holding great potential for clinical diagnosis and biomedical research studies.

中文翻译：

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通过双链特异性核酸酶辅助的靶标回收和exc受激准分子切换灵敏地检测微小RNA

在这里，我们开发了一种简单的荧光方法，用于基于双链特异性核酸酶辅助的靶标回收和pyr受激准分子转换的microRNA的灵敏检测。该方法灵敏度极高，检测极限为0.58 fM，可用于绝对检测癌细胞中的内源性microRNA，在临床诊断和生物医学研究方面具有巨大潜力。