The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins of the enhanced affinity of RNA–protein interactions triggered by the PS2 moiety.

中文翻译：

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RNA-二硫代磷酸酯骨架修饰引发的RNA-蛋白质相互作用亲和力增强的起源

MS2外壳蛋白与其同源RNA发夹之间已充分表征的相互作用被用来评估亲和力的变化，这是由于二硫代磷酸酯（PS2）通过生物层干涉术（BLI）替代了磷酸盐。对数据进行基于结构的分析，可以洞悉PS2部分触发的RNA与蛋白质相互作用的亲和力增强的起源。