The influence of nutritional regime and water temperature on depuration rates of OA-group toxins in the wedge shell Donax trunculus was examined by exposing naturally contaminated specimens to three nutritional regimes (microalgae, commercial paste of microalgae, and starvation) for 14 days at 16 °C and 20 °C. Total OA was quantified in the whole soft tissues of the individuals collected in days 2, 4, 6, 8, 10, 12 and 14. Mortality, dry weight, condition index, gross biochemical composition and gametogenic stages were surveyed. Low variation of glycogen and carbohydrates during the experiments suggest that wedge shells were under non-dramatic stress conditions. Wedge shells fed with non-toxic diets showed similar depuration rates being 15 and 38% higher than in starvation, at 16 and 20 °C, respectively. Depuration rates under non-toxic diets at 20 °C were 71% higher than at 16 °C. These results highlight the influence of water temperature on the depuration rate of total OA accumulated by D. trunculus, even when the increase is of only 4 °C, as commonly observed in week time scales in the southern Portuguese coastal waters. These results open the possibility of a faster release of OA in harvested wedge shells translocated to depuration systems when under a slight increase of water temperature.

中文翻译：

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温度和营养制度对消除天然污染的楔形壳Don的亲脂性毒素冈田酸的综合影响

营养方式和水温对楔形壳Don中OA-组毒素净化速率的影响通过在16°C和20°C下将自然污染的标本暴露于三种营养体系（微藻，微藻的商业化糊剂和饥饿）下14天来进行检测。在第2、4、6、8、10、12和14天收集的个体的整个软组织中对总OA进行定量，调查死亡率，干重，状况指数，总生化组成和配子发生阶段。实验期间糖原和碳水化合物的低变化表明楔形壳处于非剧烈的应力条件下。用无毒饲料喂养的楔壳在16和20°C时的净化率分别比饥饿时高15％和38％。在20°C下无毒饮食下的净化率比16°C下高71％。即使在葡萄牙南部沿海水域通常以周时间尺度观察到的情况下，轮枝D. trunculus，即使仅增加4°C时也是如此。这些结果打开了在水温略微升高的情况下，在转移到净化系统的已收获楔形壳中OA更快释放的可能性。