Optical fiber microprobe functionalized with Glucose oxidase (GOD) has been proposed for bio-selective and high-sensitive glucose detection. Taking advantages of the free amine groups of 3-aminopropyl- triethoxysilane (APTES), the enzymes (GOD) are immobilized on the multimode microfiber through covalent interaction. Surface characterization offered by optical microscopy, scanning electron microscope and multiphoton laser scanning microscopy provide detailed evidences about the effect of the bio-functionalization. The fabricated microfiber sensor is immersed into glucose solutions at different concentrations to record and analyze the transmission spectrums from an optical sensing interrogator. The experimental results demonstrate that the resonant wavelength shift is linearly correlated with the glucose concentration in the range of 0–3.0 mg/ml with a response coefficient of 1.74 nm/mg⁻¹ ml⁻¹. Meanwhile, the reported sensor is also proved to be of practical utility by accurately detecting glucose content in animal serum samples. Due to the small size, label-free sensing capacity and excellent practicality, the proposed device has great potential to be applied in the fields like disease diagnosis, clinical analysis and food safety.

已经提出了用葡萄糖氧化酶（GOD）功能化的光纤微探针，用于生物选择性和高灵敏度的葡萄糖检测。利用3-氨基丙基-三乙氧基硅烷（APTES）的游离胺基，通过共价相互作用将酶（GOD）固定在多模微纤维上。光学显微镜，扫描电子显微镜和多光子激光扫描显微镜提供的表面表征提供了有关生物功能化作用的详细证据。将制成的微纤维传感器浸入不同浓度的葡萄糖溶液中，以记录和分析来自光学传感询问器的透射光谱。实验结果表明，共振波长偏移与葡萄糖浓度在0–3范围内线性相关。^-1 毫升^-1。同时，通过准确检测动物血清样品中的葡萄糖含量，也证明了所报道的传感器具有实用价值。由于其体积小，无标签感应能力强和实用性强，所提出的设备在疾病诊断，临床分析和食品安全等领域具有很大的潜力。