6-O-(2-Nitrobenzyl)guanosine and 4-O-(2-nitrobenzyl)uridine triphosphates (^NBGTP, ^NBUTP) were synthesized, and their biochemical and photophysical properties were evaluated. We synthesized ^NBUTP using the canonical triphosphate synthesis method and ^NBGTP from 2′,3′-O-TBDMS guanosine via a triphosphate synthesis method by utilizing mild acidic desilylation conditions. Deprotection of the nitrobenzyl group in ^NBGTP and ^NBUTP proceeded within 60 s by UV irradiation at 365 nm. Experiments using ^NBGTP or ^NBUTP in T7-RNA transcription reactions showed that ^NBGTP could be useful for the photocontrol of transcription by UV irradiation.

6- ø - （2-硝基苄基）鸟苷和4- ø - （2-硝基苄基）尿苷三磷酸（^NB GTP，^NB UTP）的合成，和它们的生物化学和光物理性质进行了评价。我们采用规范的三磷酸合成方法合成了^NB UTP，并利用温和的酸性去甲硅烷基化条件，通过三磷酸合成方法从2'，3'- O- TBDMS鸟苷中合成了^NB GTP 。^NB GTP和^NB UTP中硝基苄基的脱保护通过365 nm的紫外线照射在60 s内进行。在T7-RNA转录反应中使用^NB GTP或^NB UTP进行的实验表明，^NB GTP可能对通过紫外线照射进行转录的光控制有用。